In vitro clonal propagation in plants is characterized by :
- Microscopy
- Northern blotting
- Electrophoresis and HPLC
- PCR and RAPD
Answer
(4) PCR and RAPD
Solution
In vitro clonal propagation (micropropagation by tissue culture) produces a large number of plants that should be genetically identical to the parent (somaclones).
⇒ The genetic uniformity (clonal fidelity) of such plants is checked with DNA-based molecular markers.
⇒ PCR-based RAPD (random amplified polymorphic DNA) gives identical band patterns for true-to-type clones and detects any somaclonal variation.
∴ In vitro clonal propagation is characterized by PCR and RAPD.
Answer: (4)