Which of the following is not required for any of the techniques of DNA fingerprinting available at present?
- DNA – DNA hybridization
- Polymerase chain reaction
- Zinc finger analysis
- Restriction enzymes
Answer
(3) Zinc finger analysis
Solution
As per NCERT, DNA fingerprinting involves isolation of DNA, digestion of DNA by restriction endonucleases, separation of fragments by electrophoresis, blotting, hybridisation using labelled VNTR probes and detection by autoradiography. PCR is used to amplify DNA when only a small sample is available.
∴ Zinc finger analysis is not part of any DNA fingerprinting technique.
Answer: (3)