You are given a tissue with its potential for differentiation in an artificial culture. Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots?
- IAA and gibberellin
- Auxin and cytokinin
- Auxin and abscisic acid
- Gibberellin and abscisic acid
Answer
(2) Auxin and cytokinin
Solution
As per NCERT, auxins promote root initiation and cytokinins promote shoot (bud) formation; in tissue culture the callus differentiates into shoots and roots depending on the ratio of auxin to cytokinin in the medium.
⇒ High cytokinin : auxin ratio shoots; high auxin : cytokinin ratio roots.
∴ Auxin and cytokinin must be added to obtain both shoots and roots.
Answer: (2)