The viability of seeds is tested by :
- 2,3,5-triphenyl tetrazolium chloride
- Safranine
- 2,6-dichlorophenol indophenol
- DMSO
Answer
(1) 2,3,5-triphenyl tetrazolium chloride
Solution
In the tetrazolium test, living (respiring) embryo tissues reduce colourless 2,3,5-triphenyl tetrazolium chloride to red formazan due to dehydrogenase activity; dead tissues remain unstained.
Safranine is a stain, 2,6-dichlorophenol indophenol is a redox dye (Hill reaction) and DMSO is a solvent / cryoprotectant.
Answer: (1)