Biotechnology: Principles and Processes · Question 43

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

1. Annealing, Denaturation, Extension
2. Denaturation, Annealing, Extension
3. Denaturation, Extension, Annealing
4. Extension, Denaturation, Annealing

Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactosidase production and the recombinant plasmid is inserted in an E. coli strain

1. It will be able to produce a novel protein with dual ability
2. It will not be able to confer ampicillin resistance to the host cell
3. The transformed cells will have the ability to resist ampicillin as well as produce -galactosidase
4. It will lead to lysis of host cell

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

1. Ligation
2. Annealing
3. Extension
4. Denaturation

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

1. Poly (A) tail sequences
2. Degenerate primer sequence
3. Okazaki sequences
4. Palindromic Nucleotide sequences

In gel electrophoresis, separated DNA fragments can be visualized with the help of

1. Ethidium bromide in infrared radiation
2. Ethidium bromide in UV radiation
3. Acetocarmine in bright blue light
4. Acetocarmine in UV radiation

Identify the wrong statement with regard to Restriction Enzymes

1. Sticky ends can be joined by using DNA ligases.
2. They cut the strand of DNA at palindromic sites.
3. Each restriction enzyme functions by inspecting the length of a DNA sequence.
4. They are useful in genetic engineering

The specific palindromic sequence which is recognized by EcoRI is

1. 5′ - GGATCC - 3′ 3′ - CCTAGG - 5′
2. 5′ - GAATTC - 3′ 3′ - CTTAAG - 5′
3. 5′ - GGAACC - 3′ 3′ - CCTTGG - 5′
4. 5′ - CTTAAG - 3′ 3′ - GAATTC - 5′

The sequence that controls the copy number of the linked DNA in the vector, is termed

1. Recognition site
2. Ori site
3. Selectable marker
4. Palindromic sequence

Match the organism with its use in biotechnology Select the correct option from the following:

1. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
2. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
3. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)

Choose the correct pair from the following

1. Exonucleases – Make cuts at specific positions within DNA
2. Ligases – Join the two DNA molecules
3. Polymerases – Break the DNA into fragments
4. Nucleases – Separate the two strands of DNA