Biotechnology and its Applications

Match List I with List II :

1. A-II, B-I, C-IV, D-III
2. A-II, B-III, C-I, D-IV
3. A-I, B-IV, C-III, D-II
4. A-I, B-II, C-IV, D-III

Arrange the following steps of somatic hybridisation in a correct sequence. Choose the correct answer from the options given below :

1. D, B, A, E, C
2. E, A, B, C, D
3. E, B, A, D, C
4. D, A, B, C, E

The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would control cotton bollworms and corn borer, respectively?

1. cryIAc and cryIIAb
2. cryIAc and cryIIIAb
3. cryIAc and cryIAb
4. cryIIAb and cryIAc

The human protein named α-1-antitrypsin, obtained from transgenic animals, is used for the treatment of .

1. Alzheimer's disease
2. Rheumatoid arthritis
3. Emphysema
4. Cystic fibrosis

The inactive form of Bt toxin is converted to the active form in the insect gut

1. by nucleases
2. due to alkaline pH
3. due to acidic pH
4. by proteases

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

1. Virus
2. Phage
3. Bacterium
4. Yeast

Silencing of specific mRNA is possible via RNAi because of

1. Complementary tRNA
2. Non-complementary ssRNA
3. Complementary dsRNA
4. Inhibitory ssRNA

Why can't insulin be given orally to diabetic patients?

1. Because of structural variation
2. Its bioavailability will be increased
3. Human body will elicit strong immune response
4. It will be digested in Gastro-Intestinal (GI) tract

Given below are two statements: In the light of the above statements, choose the correct answer from the options given below:

1. Both Statement I and Statement II are true
2. Both Statement I and Statement II are false
3. Statement I is true but Statement II is false
4. Statement I is false but Statement II is true

Match List I with List II Choose the correct answer from the options given below:

1. A-II, B-I, C-IV, D-III
2. A-III, B-I, C-II, D-IV
3. A-III, B-IV, C-I, D-II
4. A-II, B-IV, C-I, D-III

Given below are two statements: In the light of the above statements, choose the correct answer from the options given below :

1. Statement I is true but Statement II is false
2. Statement I is false but Statement II is true
3. Both Statement I and Statement II are true
4. Both Statement I and Statement II are false

Match List-I with List-II :

1. A-III, B-II, C-IV, D-I
2. A-II, B-I, C-IV, D-III
3. A-IV, B-III, C-I, D-II
4. A-II, B-IV, C-I, D-III

The Bt toxin in genetically engineered Bt cotton kills the pest by:

1. Creating pores in the midgut
2. Damaging the respiratory system
3. Degenerating the nervous system
4. Altering the pH of body fluids

Following are the steps involved in action of toxin in Bt. Cotton Choose the correct sequence of steps from the options given below:

1. E C B A D
2. B C A E D
3. A E B D C
4. B E C A D

Given below are two statements : In the light of the above statements, choose the correct answer from the options given below.

1. Statement I is true but Statement II is false.
2. Statement I is false but Statement II is true.
3. Both Statement I and Statement II are true.
4. Both Statement I and Statement II are false.

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

1. Serum and Urine analysis
2. Polymerase Chain Reaction (PCR) technique
3. Enzyme Linked Immuno-Sorbent Assay (ELISA) technique
4. Recombinant DNA Technology

Transposons can be used during which one of the following?

1. Polymerase Chain Reaction
2. Gene Silencing
3. Autoradiography
4. Gene sequencing

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

1. Retroviral vector is introduced into these lymphocytes.
2. Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages
3. Lymphocytes from patient's blood are grown in culture, outside the body
4. Genetically engineered lymphocytes are not immortal cells.

Statements related to human Insulin are given below. Which statements is/are correct about genetically engineered Insulin? Choose the most appropriate answer from the options given below :

1. A, B and D only
2. B only
3. C and D only
4. C, D and E only

Milk of transgenic ‘Cow Rosie’ was nutritionally more balanced product for human babies than natural cow milk because it contained:

1. Human enzyme Adenosine Deaminase (ADA)
2. Human protein α-1-antitrypsin
3. Human alpha-lactalbumin
4. Human insulin-like growth factor

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :

1. Safety testing
2. Biopiracy
3. Gene therapy
4. Molecular diagnosis

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

1. Detection of gene mutation
2. Molecular diagnosis
3. Gene amplification
4. Purification of isolated protein

Now-a-days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise to its complementary DNA in a clone of cells, followed by its detection using autoradiography because:

1. mutated gene does not appear on photographic film as the probe has complementarity with it
2. mutated gene partially appears on a photographic film
3. mutated gene completely and clearly appears on a photographic film
4. mutated gene does not appear on a photographic film as the probe has no complementarity with it

With regard to insulin choose correct options. Choose the correct answer from the options given below.

1. A and D only
2. B and D only
3. B and C only
4. A, C and D only

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

1. Hybridization Technique
2. Western Blotting Technique
3. Southern Blotting Technique
4. ELISA Technique

The Adenosine deaminase deficiency results into

1. Addison's disease
2. Dysfunction of immune system
3. Parkinson's disease
4. Digestive disorder

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

1. Insect predators
2. Insect pests
3. Fungal diseases
4. Plant nematodes

Which of the following statements is not correct?

1. In man insulin is synthesised as a proinsulin
2. The proinsulin has an extra peptide called C-peptide.
3. Genetically engineered insulin is produced in E. coli.
4. The functional insulin has A and B chains linked together by hydrogen bonds.

Match the following columns and select the correct option.

1. (a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(iv), (b)-(i), (c)-(ii), (d)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)

What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?

1. Acidic pH of stomach
2. Body temperature
3. Alkaline pH of gut
4. Moist surface of midgut