Arrange the following steps of somatic hybridisation in a correct sequence. Choose the correct answer from the options given below :
- D, B, A, E, C
- E, A, B, C, D
- E, B, A, D, C
- D, A, B, C, E
Answer
(4) D, A, B, C, E
Solution
Steps of somatic hybridisation:
D ⇒ single cells are isolated from the two different varieties of plants.
A ⇒ their cell walls are digested (by pectinase and cellulase).
B ⇒ naked protoplasts are isolated.
C ⇒ protoplasts of the two varieties are fused to get a hybrid protoplast.
E ⇒ the hybrid protoplast is grown to form a new plant (somatic hybrid, e.g. pomato).
∴ Correct sequence: D, A, B, C, E
Answer: (4)