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Molecular Basis of Inheritance · Question 94

AIPMT 2014MCQEasy📈 Topic in 25% papers+4−1
1

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

  1. Blotting
  2. PCR
  3. Electrophoresis
  4. Autoradiography

Answer

(2) PCR

Solution

As per NCERT, the steps of DNA fingerprinting (Southern blot hybridisation) are:

(i) isolation of DNA, (ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis, (iv) transferring (blotting) of separated DNA fragments to synthetic membranes such as nitrocellulose or nylon, (v) hybridisation using a labelled VNTR probe, and (vi) detection of hybridised DNA fragments by autoradiography.

∴ Blotting, electrophoresis and autoradiography are used, but PCR is not a step of Southern hybridisation.

Answer: (2)

AIPMT 2014

Keep practising — Molecular Basis of Inheritance

2

Commonly used vectors for human genome sequencing are :

  1. BAC and YAC
  2. T/A Cloning Vectors
  3. T-DNA
  4. Expression Vectors
AIPMT 2014
3

The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C. [figure]

  1. A-translation B-extension C-Rosalind Franklin
  2. A-translation B-transcription C-Erwin Chargaff
  3. A-transcription B-translation C-Francis Crick
  4. A-transcription B-replication C-James Watson
NEET 2013
4

Which enzyme will be produced in a cell in which there is a non-sense mutation in the lac Y gene ?

  1. Transacetylase
  2. Lactose permease and transacetylase
  3. Lactose permease
  4. -galactosidase
NEET 2013
5

[figure] The figure gives an important concept in the genetic implication of DNA. Fill the blanks A, B and C.

  1. A - Erwin Chargaff B - translation C - replication
  2. A - Maurice Wilkins B - transcription C - translation
  3. A - Francis Crick B - translation C - transcription
  4. A - James Watson B - replication C - extension
NEET 2013 (Karnataka)
6

Which of the following is not a property of the genetic code ?

  1. Ambiguous
  2. Universal
  3. Degeneracy
  4. Non-overlapping
NEET 2013 (Karnataka)
7

In an inducible operon, the genes are :

  1. usually expressed unless a signal turns them "off"
  2. always expressed
  3. never expressed
  4. usually not expressed unless a signal turns them "on"
NEET 2013 (Karnataka)
8

One of the most frequently used techniques in DNA fingerprinting is :-

  1. SSCP
  2. SCAR
  3. VNTR
  4. AFLP
NEET 2013 (Karnataka)
9

Read the following four statements (A-D) : How many of the above statements are right ?

  1. Four
  2. Two
  3. Three
  4. One
AIPMT 2012 (Mains)
10

What is it that forms the basis of DNA Fingerprinting ?

  1. The relative proportions of purines and pyrimidines in DNA
  2. The relative amount of DNA in the ridges and grooves of the fingerprints
  3. The relative difference in the DNA occurrence in blood, skin and saliva
  4. Satellite DNA occurring as highly repeated short DNA segments
AIPMT 2012 (Mains)
11

Removal of introns and joining of exons in a defined order during transcription is called

  1. Looping
  2. Inducing
  3. Slicing
  4. Splicing
AIPMT 2012 (Prelims)

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