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Biotechnology: Principles and Processes · Question 114

AIPMT 2000MCQEasy📈 Topic in 88% papers+4−1
1

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus

Answer

(3) Agrobacterium

Solution

As per NCERT, Agrobacterium tumefaciens, a pathogen of dicot plants, delivers a piece of DNA (T-DNA) into plant cells.

⇒ Its tumour-inducing (Ti) plasmid has been modified into a cloning vector that is no longer pathogenic but still delivers genes of interest into plants.

∴ Agrobacterium is the bacterium generally used for genetic engineering of plants.

Answer: (3)

AIPMT 2000

Keep practising — Biotechnology: Principles and Processes

2

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells
AIPMT 2000

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