You are required to draw blood from a patient and to keep it in a test tube for analysis of blood corpuscles and plasma. You are also provided with the following four types of test tubes. Which of these will you not use for the purpose?
- Test tube containing calcium bicarbonate
- Chilled test tube
- Test tube containing heparin
- Test tube containing sodium oxalate.
Answer
(1) Test tube containing calcium bicarbonate
Solution
Blood kept for analysis of corpuscles and plasma must be prevented from clotting.
As per NCERT, calcium ions play a very important role in clotting (coagulation) of blood.
⇒ A tube containing calcium bicarbonate adds Ca2+ ions, so the blood will clot; this tube cannot be used.
∵ Heparin is an anticoagulant, sodium oxalate removes Ca2+ as insoluble calcium oxalate, and chilling slows the enzymatic clotting reactions, the other three tubes keep the blood unclotted.
∴ The test tube containing calcium bicarbonate will not be used.
Answer: (1)