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Biotechnology: Principles and Processes · Question 72

AIPMT 2015MCQEasy📈 Topic in 88% papers+4−1
1

The introduction of T-DNA into plants involves -

  1. Altering the pH of the soil, then heat-shocking the plants
  2. Infection of the plant by Agrobacterium tumefaciens
  3. Exposing the plants to cold for a brief period
  4. Allowing the plant roots to stand in water

Answer

(2) Infection of the plant by Agrobacterium tumefaciens

Solution

As per NCERT, Agrobacterium tumefaciens, a pathogen of several dicot plants, is able to deliver a piece of DNA known as T-DNA to transform normal plant cells into a tumor.

⇒ T-DNA is a part of the Ti plasmid of Agrobacterium tumefaciens; it enters the plant only when the bacterium infects the plant.

Answer: (2)

AIPMT 2015

Keep practising — Biotechnology: Principles and Processes

2

Which vector can clone only a small fragment of DNA ?

  1. Cosmid
  2. Bacterial artificial chromosome
  3. Yeast artificial chromosome
  4. Plasmid
AIPMT 2014
3

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

  1. Insertional inactivation of alphagalactosidase in recombinant bacteria
  2. Inactivation of glycosidase enzyme in recombinant bacteria
  3. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
  4. Non-recombinant bacteria containing betagalactosidase
NEET 2013
4

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

  1. Polymerase chain reaction
  2. Restriction mapping
  3. Electrophoresis
  4. Centrifugation
NEET 2013
5

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?

  1. Plant cells- Cellulase
  2. Algae-Methylase
  3. Fungi - Chitinase
  4. Bacteria-Lysozyme
NEET 2013
6

Genes of interest can be selected from a genomic library by using :

  1. Restriction enzymes
  2. Gene targets
  3. Cloning vectors
  4. DNA probes
NEET 2013 (Karnataka)
7

During the process of isolation of DNA, chilled ethanol is added to :

  1. Break open the cell to release DNA
  2. Remove proteins such as histones
  3. Facilitate action of restriction enzymes
  4. Precipitate DNA
NEET 2013 (Karnataka)
8

Biolistics (gene-gun) is suitable for

  1. DNA finger printing
  2. Constructing recombinant DNA by joining with vectors
  3. Disarming pathogen vectors
  4. Transformation of plant cells
AIPMT 2012 (Mains)
9

In genetic engineering, the antibiotics are used

  1. As sequences from where replication starts
  2. As selectable markers
  3. To keep the cultures free of infection
  4. To select healthy vectors
AIPMT 2012 (Mains)
10

Which one of the following represents a palindromic sequence in DNA ?

  1. 5′-GAATTC-3′ 3′-CTTAAG-5′
  2. 5′-GATACC-3′ 3′-CCTAAG-5′
  3. 5′-CCAATG-3′ 3′-GAATCC-5′
  4. 5′-CATTAG-3′ 3′-GATAAC-5′
AIPMT 2012 (Mains)
11

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents ? [figure]

  1. C - Extension in the presence of heat stable DNA polymerase
  2. B - Denaturation at a temperature of about 98 separating the two DNA strands
  3. A - Denaturation at a temperature of about 50
  4. A - Annealing with two sets of primers
AIPMT 2012 (Mains)

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