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Biotechnology: Principles and Processes · Question 82

AIPMT 2012 (Mains)MCQMedium📈 Topic in 88% papers+4−1
1

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents ? [figure]

  1. C - Extension in the presence of heat stable DNA polymerase
  2. B - Denaturation at a temperature of about 98 separating the two DNA strands
  3. A - Denaturation at a temperature of about 50
  4. A - Annealing with two sets of primers

Answer

(1) C - Extension in the presence of heat stable DNA polymerase

Solution

In the figure: A = the double-stranded DNA (template) with the region to be amplified; B = single strands with short primers (black) bound — annealing; C = primers extended along the whole region — extension.

As per NCERT, in extension the primers are extended using the nucleotides and the thermostable DNA polymerase (isolated from Thermus aquaticus, Taq polymerase).

Denaturation (about 94–98 ) separates the strands; it is not shown as B and does not occur at 50 ; A is not annealing.

∴ C – Extension in the presence of heat stable DNA polymerase.

Answer: (1)

AIPMT 2012 (Mains)

Keep practising — Biotechnology: Principles and Processes

2

The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? [figure]

  1. ori - original restriction enzyme
  2. ampR, tetR - antibiotic resistance genes
  3. rop - reduced osmotic pressure
  4. Hind III, EcoR I - selectable markers
AIPMT 2012 (Prelims)
3

Which one is a true statement regarding DNA polymerase used in PCR?

  1. It is used to ligate introduced DNA in recipient cell
  2. It remains active at high temperature
  3. It serves as a selectable marker
  4. It is isolated from a virus
AIPMT 2012 (Prelims)
4

For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of

  1. Gold or tungsten
  2. Platinum or zinc
  3. Silver or platinum
  4. Silicon or platinum
AIPMT 2012 (Prelims)
5

Which one of the following techniques made it possible to genetically engineer living organisms?

  1. Recombinant DNA techniques
  2. X-ray diffraction
  3. Heavier isotope labeling
  4. Hybridization
AIPMT 2011 (Mains)
6

Read the following four statements (A-D) with certain mistakes in two of them. Which are the two statements having mistakes?

  1. Statements (B) and (C)
  2. Statements (A) and (C)
  3. Statements (A) and (B)
  4. Statements (C) and (D)
AIPMT 2011 (Mains)
7

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it ? 5' .............. GAATTC ................ 3' 3' .............. CTTAAG ................ 5'

  1. Start codon at the 5' end
  2. Replication completed
  3. Deletion mutation
  4. Palindromic sequence of base pairs
AIPMT 2011 (Prelims)
8

There is a restriction endonuclease called EcoRI. What does "co" part in it stand for ?

  1. colon
  2. coelom
  3. coenzyme
  4. coli
AIPMT 2011 (Prelims)
9

Agarose extracted from sea weeds finds use in :

  1. Spectrophotometry
  2. PCR
  3. Tissue Culture
  4. Gel electrophoresis
AIPMT 2011 (Prelims)
10

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector / vectors –

  1. B and D only
  2. A and C only
  3. A, B and D only
  4. A only
AIPMT 2010 (Mains)
11

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

  1. 5′ —— CGTTCG —— 3′ 3′ —— ATGGTA —— 5′
  2. 5′ —— GAATTC —— 3′ 3′ —— CTTAAG —— 5′
  3. 5′ —— CACGTA —— 3′ 3′ —— CTCAGT —— 5′
  4. 5′ —— GATATG —— 3′ 3′ —— CTACTA —— 5′
AIPMT 2010 (Prelims)

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