Arrange the following steps of DNA fingerprinting in a correct sequence. Choose the correct answer from the options given below :
- A, E, B, C, D
- A, D, B, E, C
- A, B, D, C, E
- A, E, C, B, D
Answer
(4) A, E, C, B, D
Solution
As per NCERT, DNA fingerprinting involves:
A ⇒ isolation of DNA and its digestion by restriction endonucleases
E ⇒ separation of DNA fragments by electrophoresis
C ⇒ transferring (blotting) the separated fragments to synthetic membranes (nitrocellulose or nylon)
B ⇒ hybridisation using a labelled VNTR probe
D ⇒ detection of hybridised DNA fragments by autoradiography
∴ Correct sequence: A, E, C, B, D
Answer: (4)