Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used -
- On plates with minimal medium
- Only on plates with streptomycin
- Only on plates without streptomycin
- On plates with and without streptomycin
Answer
(2) Only on plates with streptomycin
Solution
This is the replica plating experiment of Lederberg and Lederberg.
⇒ Colonies on the master plate (complete medium, no streptomycin) are copied with sterile velvet onto plates containing streptomycin.
⇒ Only the resistant colonies grow; these occupy the same positions as particular colonies on the master plate, which were never exposed to the drug.
∴ Resistance arose by random mutation before contact with streptomycin, not as an adaptation to it. As per NCERT, variations (mutations) are random and pre-existing, and selection only picks them.
Answer: (2)