Biotechnology: Principles and Processes · Question 22

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.

1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI

Which of the following are correct about EcoRI? Choose the correct answer from the options given below:

1. B, C, E only
2. A, D, E only
3. A, C, D only
4. B, C, D only

Following are the steps involved in the process of PCR. Choose the correct sequence of steps of PCR from the options given below :

1. C A D E B
2. A B E D C
3. A C E D B
4. D B E C A

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

1. DNA
2. Histones
3. Polysaccharides
4. RNA

In gene gun method used to introduce alien DNA into host cells, microparticles of metal are used.

1. Zinc
2. Tungsten or gold
3. Silver
4. Copper

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

1. Bright blue colour
2. Bright yellow colour
3. Bright orange colour
4. Bright red colour

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. Choose the correct answer from the options given below :

1. C, A, B, D
2. C, B, D, A
3. B, D, A, C
4. B, C, D, A

Which of the following is not a cloning vector?

1. YAC
2. pBR322
3. Probe
4. BAC

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). In the light of the above statements, choose the correct answer from the options given below :

1. Both (A) and (R) are correct and (R) is the correct explanation of (A)
2. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
3. (A) is correct but (R) is not correct
4. (A) is not correct but (R) is correct

Which one of the following statements is not true regarding gel electrophoresis technique?

1. The process of extraction of separated DNA strands from gel is called elution.
2. The separated DNA fragments are stained by using ethidium bromide.
3. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
4. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.