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Biotechnology: Principles and Processes · Question 32

NEET (UG) 2022MCQEasy📈 Topic in 88% papers+4−1
1

Which one of the following statements is not true regarding gel electrophoresis technique?

  1. The process of extraction of separated DNA strands from gel is called elution.
  2. The separated DNA fragments are stained by using ethidium bromide.
  3. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
  4. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.

Answer

(3) The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

Solution

As per NCERT, the separated DNA fragments are visualised only after staining with ethidium bromide followed by exposure to UV radiation; they appear as bright orange coloured bands ⇒ (2) and (4) are true.

The separated bands are cut out from the agarose gel and extracted from the gel piece; this step is called elution ⇒ (1) is true.

A chromogenic substrate gives blue coloured colonies of bacteria (in insertional inactivation of the β-galactosidase gene), not blue DNA bands on the gel ⇒ (3) is not true.

Answer: (3)

NEET (UG) 2022

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2

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

  1. 5'GATACT3' ; 3'CTATGA5'
  2. 5'GAATTC3' ; 3'CTTAAG5'
  3. 5'CTCAGT3' ; 3'GAGTCA5'
  4. 5'GTATTC3' ; 3'CATAAG5'
NEET (UG) 2022
3

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below :

  1. Both Statement I and Statement II are correct
  2. Both Statement I and Statement II are incorrect
  3. Statement I is correct but Statement II is incorrect
  4. Statement I is incorrect but Statement II is correct
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4

Which of the following is not a desirable feature of a cloning vector?

  1. Presence of origin of replication
  2. Presence of a marker gene
  3. Presence of single restriction enzyme site
  4. Presence of two or more recognition sites
NEET (UG) 2022
5

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). In the light of the above statements, choose the correct answer from the options given below :

  1. (A) is not correct but (R) is correct
  2. Both (A) and (R) are correct and (R) is the correct explanation of (A)
  3. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
  4. (A) is correct but (R) is not correct
NEET (UG) 2022 (Phase 2)
6

Separation of DNA fragments is done by a technique known as

  1. Gel electrophoresis
  2. Polymerase Chain Reaction
  3. Recombinant technology
  4. Southern blotting
NEET (UG) 2022 (Phase 2)
7

The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :

  1. (a) Cellulase (b) Lipase
  2. (a) Cellulase (b) Protease
  3. (a) Cellulase (b) Chitinase
  4. (a) Chitinase (b) Lipase
NEET (UG) 2022 (Phase 2)
8

Match List-I with List-II : Choose the correct answer from the options given below:

  1. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
  2. (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
  3. (a)-(i), (b)-(iii), (c)-(ii), (d)-(iv)
  4. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
NEET (UG) 2022 (Phase 2)
9

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

  1. Bacteriophages
  2. Agrobacterium mediated transformation
  3. Gene gun
  4. ‘Disarmed pathogen’ vectors
NEET (UG) 2022 (Phase 2)
10

Refer to the following statements for agarose-gel electrophoresis: Choose the most appropriate answer from the options given below :

  1. B, C and D only
  2. A and B only
  3. A, B and C only
  4. A, B and D only
NEET (UG) 2022 (Phase 2)
11

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

  1. Polysaccharides
  2. RNA
  3. DNA
  4. Histones
NEET (UG) 2021

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