All enzymes of TCA cycle are located in the mitochondrial matrix except one which is located in inner mitochondrial membrane in eukaryotes and in cytosol in prokaryotes. This enzyme is
- isocitrate dehydrogenase
- malate dehydrogenase
- succinate dehydrogenase
- lactate dehydrogenase
Answer
(3) succinate dehydrogenase
Solution
Succinate dehydrogenase, which oxidises succinate to fumarate (with FAD → FADH2), is the only TCA-cycle enzyme bound to the inner mitochondrial membrane; it is also complex II of the electron transport system. In prokaryotes (no mitochondria) it is present in the cytosol / plasma membrane.
∵ Isocitrate dehydrogenase and malate dehydrogenase are in the matrix, and lactate dehydrogenase is not a TCA-cycle enzyme (it acts in lactic acid fermentation) ⇒ (1), (2), (4) are wrong.
Answer: (3)