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Biotechnology: Principles and Processes · Question 70

AIPMT 2015MCQEasy📈 Topic in 88% papers+4−1
1

The DNA molecule to which the gene of interest is integrated for cloning is called -

  1. Transformer
  2. Template
  3. Vector
  4. Carrier

Answer

(3) Vector

Solution

As per NCERT, the gene of interest (alien DNA) is linked to a DNA molecule that can replicate inside the host cell; this carrier DNA molecule is called a vector (e.g. plasmids, bacteriophages).

⇒ The vector carries the gene into the host and multiplies it along with its own DNA.

Answer: (3)

AIPMT 2015

Keep practising — Biotechnology: Principles and Processes

2

The cutting of DNA at specific locations became possible with the discovery of -

  1. Restriction enzymes
  2. Selectable markers
  3. Probes
  4. Ligases
AIPMT 2015
3

The introduction of T-DNA into plants involves -

  1. Altering the pH of the soil, then heat-shocking the plants
  2. Infection of the plant by Agrobacterium tumefaciens
  3. Exposing the plants to cold for a brief period
  4. Allowing the plant roots to stand in water
AIPMT 2015
4

Which vector can clone only a small fragment of DNA ?

  1. Cosmid
  2. Bacterial artificial chromosome
  3. Yeast artificial chromosome
  4. Plasmid
AIPMT 2014
5

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

  1. Insertional inactivation of alphagalactosidase in recombinant bacteria
  2. Inactivation of glycosidase enzyme in recombinant bacteria
  3. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
  4. Non-recombinant bacteria containing betagalactosidase
NEET 2013
6

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

  1. Polymerase chain reaction
  2. Restriction mapping
  3. Electrophoresis
  4. Centrifugation
NEET 2013
7

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?

  1. Plant cells- Cellulase
  2. Algae-Methylase
  3. Fungi - Chitinase
  4. Bacteria-Lysozyme
NEET 2013
8

Genes of interest can be selected from a genomic library by using :

  1. Restriction enzymes
  2. Gene targets
  3. Cloning vectors
  4. DNA probes
NEET 2013 (Karnataka)
9

During the process of isolation of DNA, chilled ethanol is added to :

  1. Break open the cell to release DNA
  2. Remove proteins such as histones
  3. Facilitate action of restriction enzymes
  4. Precipitate DNA
NEET 2013 (Karnataka)
10

Biolistics (gene-gun) is suitable for

  1. DNA finger printing
  2. Constructing recombinant DNA by joining with vectors
  3. Disarming pathogen vectors
  4. Transformation of plant cells
AIPMT 2012 (Mains)
11

In genetic engineering, the antibiotics are used

  1. As sequences from where replication starts
  2. As selectable markers
  3. To keep the cultures free of infection
  4. To select healthy vectors
AIPMT 2012 (Mains)

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