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Biotechnology: Principles and Processes · Question 69

NEET 2016 (Phase 2)MCQMedium📈 Topic in 88% papers+4−1
1

Which of the following restriction enzymes produces blunt ends ?

  1. Eco RV
  2. Hind III
  3. Xho I
  4. Sal I

Answer

(1) Eco RV

Solution

Restriction enzymes that cut both strands of DNA at the same point (straight across) give blunt ends; those that cut the two strands a little away from each other give sticky ends.

⇒ Eco RV cuts at the centre of its recognition site GAT↓ATC on both strands, so it produces blunt ends.

∵ Hind III (A↓AGCTT), Xho I (C↓TCGAG) and Sal I (G↓TCGAC) make staggered cuts, they produce sticky (cohesive) ends.

Answer: (1)

NEET 2016 (Phase 2)

Keep practising — Biotechnology: Principles and Processes

2

The DNA molecule to which the gene of interest is integrated for cloning is called -

  1. Transformer
  2. Template
  3. Vector
  4. Carrier
AIPMT 2015
3

The cutting of DNA at specific locations became possible with the discovery of -

  1. Restriction enzymes
  2. Selectable markers
  3. Probes
  4. Ligases
AIPMT 2015
4

The introduction of T-DNA into plants involves -

  1. Altering the pH of the soil, then heat-shocking the plants
  2. Infection of the plant by Agrobacterium tumefaciens
  3. Exposing the plants to cold for a brief period
  4. Allowing the plant roots to stand in water
AIPMT 2015
5

Which vector can clone only a small fragment of DNA ?

  1. Cosmid
  2. Bacterial artificial chromosome
  3. Yeast artificial chromosome
  4. Plasmid
AIPMT 2014
6

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

  1. Insertional inactivation of alphagalactosidase in recombinant bacteria
  2. Inactivation of glycosidase enzyme in recombinant bacteria
  3. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
  4. Non-recombinant bacteria containing betagalactosidase
NEET 2013
7

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

  1. Polymerase chain reaction
  2. Restriction mapping
  3. Electrophoresis
  4. Centrifugation
NEET 2013
8

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?

  1. Plant cells- Cellulase
  2. Algae-Methylase
  3. Fungi - Chitinase
  4. Bacteria-Lysozyme
NEET 2013
9

Genes of interest can be selected from a genomic library by using :

  1. Restriction enzymes
  2. Gene targets
  3. Cloning vectors
  4. DNA probes
NEET 2013 (Karnataka)
10

During the process of isolation of DNA, chilled ethanol is added to :

  1. Break open the cell to release DNA
  2. Remove proteins such as histones
  3. Facilitate action of restriction enzymes
  4. Precipitate DNA
NEET 2013 (Karnataka)
11

Biolistics (gene-gun) is suitable for

  1. DNA finger printing
  2. Constructing recombinant DNA by joining with vectors
  3. Disarming pathogen vectors
  4. Transformation of plant cells
AIPMT 2012 (Mains)

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