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Biotechnology and its Applications · Question 72

AIPMT 2004MCQMedium📈 Topic in 12% papers+4−1
1

ELISA is used to detect viruses, where the key reagent is :-

  1. Catalase
  2. RNase
  3. DNA probes
  4. Alkaline phosphatase

Answer

(4) Alkaline phosphatase

Solution

ELISA (Enzyme Linked Immunosorbent Assay) works on the principle of antigen–antibody interaction.

⇒ The detecting (secondary) antibody is linked to an enzyme, commonly alkaline phosphatase (or peroxidase).

⇒ The enzyme acts on its substrate to give a coloured product, which shows the presence of the viral antigen.

∴ The key reagent is alkaline phosphatase.

Answer: (4)

AIPMT 2004

Keep practising — Biotechnology and its Applications

2

Maximum application of animal cell culture technology today is in the production of :-

  1. Interferons
  2. Vaccines
  3. Edible proteins
  4. Insulin
AIPMT 2003
3

ELISA is used to detect viruses, where the key reagent is :-

  1. Alkaline phosphatase
  2. RNase
  3. DNA probes
  4. Catalase
AIPMT 2003

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