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Biotechnology and its Applications · Question 74

AIPMT 2003MCQMedium📈 Topic in 12% papers+4−1
1

ELISA is used to detect viruses, where the key reagent is :-

  1. Alkaline phosphatase
  2. RNase
  3. DNA probes
  4. Catalase

Answer

(1) Alkaline phosphatase

Solution

As per NCERT, ELISA is based on the principle of antigen–antibody interaction; infection by a pathogen can be detected by the presence of antigens or of antibodies synthesised against the pathogen.

⇒ In ELISA the detecting antibody is linked to an enzyme; on adding its substrate, the enzyme gives a coloured product whose intensity shows the amount of antigen.

⇒ The commonly used enzymes are alkaline phosphatase and horseradish peroxidase.

∴ The key reagent is alkaline phosphatase. (DNA probes are used in hybridisation methods, not in ELISA.)

Answer: (1)

AIPMT 2003

Keep practising — Biotechnology and its Applications

2

Match List I with List II :

  1. A-II, B-I, C-IV, D-III
  2. A-II, B-III, C-I, D-IV
  3. A-I, B-IV, C-III, D-II
  4. A-I, B-II, C-IV, D-III
NEET (UG) 2026
3

Arrange the following steps of somatic hybridisation in a correct sequence. Choose the correct answer from the options given below :

  1. D, B, A, E, C
  2. E, A, B, C, D
  3. E, B, A, D, C
  4. D, A, B, C, E
NEET (UG) 2026
4

The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would control cotton bollworms and corn borer, respectively?

  1. cryIAc and cryIIAb
  2. cryIAc and cryIIIAb
  3. cryIAc and cryIAb
  4. cryIIAb and cryIAc
NEET (UG) 2026
5

The human protein named α-1-antitrypsin, obtained from transgenic animals, is used for the treatment of .

  1. Alzheimer's disease
  2. Rheumatoid arthritis
  3. Emphysema
  4. Cystic fibrosis
NEET (UG) 2026
6

The inactive form of Bt toxin is converted to the active form in the insect gut

  1. by nucleases
  2. due to alkaline pH
  3. due to acidic pH
  4. by proteases
RE-NEET (UG) 2026
7

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

  1. Virus
  2. Phage
  3. Bacterium
  4. Yeast
NEET (UG) 2025
8

Silencing of specific mRNA is possible via RNAi because of

  1. Complementary tRNA
  2. Non-complementary ssRNA
  3. Complementary dsRNA
  4. Inhibitory ssRNA
NEET (UG) 2025
9

Why can't insulin be given orally to diabetic patients?

  1. Because of structural variation
  2. Its bioavailability will be increased
  3. Human body will elicit strong immune response
  4. It will be digested in Gastro-Intestinal (GI) tract
NEET (UG) 2025
10

Given below are two statements: In the light of the above statements, choose the correct answer from the options given below:

  1. Both Statement I and Statement II are true
  2. Both Statement I and Statement II are false
  3. Statement I is true but Statement II is false
  4. Statement I is false but Statement II is true
NEET (UG) 2024
11

Match List I with List II Choose the correct answer from the options given below:

  1. A-II, B-I, C-IV, D-III
  2. A-III, B-I, C-II, D-IV
  3. A-III, B-IV, C-I, D-II
  4. A-II, B-IV, C-I, D-III
NEET (UG) 2024

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