Biotechnology: Principles and Processes · Question 36

Separation of DNA fragments is done by a technique known as

1. Gel electrophoresis
2. Polymerase Chain Reaction
3. Recombinant technology
4. Southern blotting

The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :

1. (a) Cellulase (b) Lipase
2. (a) Cellulase (b) Protease
3. (a) Cellulase (b) Chitinase
4. (a) Chitinase (b) Lipase

Match List-I with List-II : Choose the correct answer from the options given below:

1. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
2. (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
3. (a)-(i), (b)-(iii), (c)-(ii), (d)-(iv)
4. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

1. Bacteriophages
2. Agrobacterium mediated transformation
3. Gene gun
4. ‘Disarmed pathogen’ vectors

Refer to the following statements for agarose-gel electrophoresis: Choose the most appropriate answer from the options given below :

1. B, C and D only
2. A and B only
3. A, B and C only
4. A, B and D only

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

1. Polysaccharides
2. RNA
3. DNA
4. Histones

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as

1. Bright blue bands
2. Yellow bands
3. Bright orange bands
4. Dark red bands

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

1. Annealing, Denaturation, Extension
2. Denaturation, Annealing, Extension
3. Denaturation, Extension, Annealing
4. Extension, Denaturation, Annealing

Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactosidase production and the recombinant plasmid is inserted in an E. coli strain

1. It will be able to produce a novel protein with dual ability
2. It will not be able to confer ampicillin resistance to the host cell
3. The transformed cells will have the ability to resist ampicillin as well as produce -galactosidase
4. It will lead to lysis of host cell

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

1. Ligation
2. Annealing
3. Extension
4. Denaturation