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Biotechnology: Principles and Processes · Question 66

NEET 2016 (Phase 2)MCQEasy📈 Topic in 50% papers+4−1
1

Stirred-tank bioreactors have been designed for -

  1. ensuring anaerobic conditions in the culture vessel
  2. addition of preservatives to the product
  3. purification of product
  4. availability of oxygen throughout the process

Answer

(4) availability of oxygen throughout the process

Solution

As per NCERT, the most commonly used bioreactors are of the stirring type. A stirred-tank reactor is usually cylindrical or with a curved base to facilitate mixing of the reactor contents.

⇒ The stirrer facilitates even mixing and oxygen availability throughout the bioreactor; alternatively air can be bubbled through the reactor (sparged stirred-tank reactor).

⇒ Purification and addition of preservatives are steps of downstream processing, not functions of the bioreactor.

Answer: (4)

NEET 2016 (Phase 2)

Keep practising — Biotechnology: Principles and Processes

2

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

  1. Eco RI
  2. Polymerase III
  3. Ligase
  4. Taq polymerase
NEET 2016 (Phase 2)
3

Which of the following is not a component of downstream processing ?

  1. Purification
  2. Expression
  3. Preservation
  4. Separation
NEET 2016 (Phase 2)
4

Which of the following restriction enzymes produces blunt ends ?

  1. Eco RV
  2. Hind III
  3. Xho I
  4. Sal I
NEET 2016 (Phase 2)
5

The DNA molecule to which the gene of interest is integrated for cloning is called -

  1. Transformer
  2. Template
  3. Vector
  4. Carrier
AIPMT 2015
6

The cutting of DNA at specific locations became possible with the discovery of -

  1. Restriction enzymes
  2. Selectable markers
  3. Probes
  4. Ligases
AIPMT 2015
7

The introduction of T-DNA into plants involves -

  1. Altering the pH of the soil, then heat-shocking the plants
  2. Infection of the plant by Agrobacterium tumefaciens
  3. Exposing the plants to cold for a brief period
  4. Allowing the plant roots to stand in water
AIPMT 2015
8

Which vector can clone only a small fragment of DNA ?

  1. Cosmid
  2. Bacterial artificial chromosome
  3. Yeast artificial chromosome
  4. Plasmid
AIPMT 2014
9

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

  1. Insertional inactivation of alphagalactosidase in recombinant bacteria
  2. Inactivation of glycosidase enzyme in recombinant bacteria
  3. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
  4. Non-recombinant bacteria containing betagalactosidase
NEET 2013
10

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

  1. Polymerase chain reaction
  2. Restriction mapping
  3. Electrophoresis
  4. Centrifugation
NEET 2013
11

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?

  1. Plant cells- Cellulase
  2. Algae-Methylase
  3. Fungi - Chitinase
  4. Bacteria-Lysozyme
NEET 2013

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