Biotechnology: Principles and Processes · Question 1

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

1. Denaturation Extension Annealing
2. Denaturation Annealing Extension
3. Annealing Denaturation Extension
4. Extension Annealing Denaturation

Which of the following statements are correct with respect to DNA separation, isolation and visualization? Choose the correct answer from the options given below :

1. A and B only
2. B and D only
3. A and D only
4. B and C only

Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards :

1. Ampicillin and tetracycline
2. Ampicillin
3. Tetracycline
4. Gentamycin

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). In the light of the above statements, choose the most appropriate answer from the options given below:

1. A is not correct but R is correct
2. Both A and R are correct and R is the correct explanation of A
3. Both A and R are correct but R is not the correct explanation of A
4. A is correct but R is not correct

Match List-I with List-II.

1. A-IV, B-I, C-III, D-II
2. A-II, B-I, C-IV, D-III
3. A-I, B-II, C-IV, D-III
4. A-III, B-IV, C-II, D-I

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below:

1. Statement I is incorrect but Statement II is correct
2. Both Statement I and Statement II are correct
3. Both Statement I and Statement II are incorrect
4. Statement I is correct but Statement II is incorrect

During PCR, primers bind to the DNA strands in the step.

1. ligation
2. denaturation
3. extension
4. annealing

[figure] In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

1. White color colonies will be selected.
2. Blue color colonies grown on ampicillin plates can be selected.
3. Using ampicillin & tetracycline containing medium plate.
4. Blue color colonies will be selected.

Polymerase chain reaction (PCR) amplifies DNA following the equation.

1. 2n+1
2. 2 N2
3. N2
4. 2n

Identify the part of a bio-reactor which is used as a foam breaker from the given figure. [figure]

1. D
2. C
3. A
4. B