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Biotechnology: Principles and Processes · Question 57

NEET 2018MCQEasy📈 Topic in 88% papers+4−1
1

The correct order of steps in Polymerase Chain Reaction (PCR) is

  1. extension, denaturation, annealing
  2. annealing, extension, denaturation
  3. denaturation, extension, annealing
  4. denaturation, annealing, extension

Answer

(4) denaturation, annealing, extension

Solution

As per NCERT, each PCR cycle has three steps:

(i) Denaturation: the double-stranded DNA is separated into single strands at high temperature.

(ii) Annealing: two sets of primers bind to the complementary regions of the single strands.

(iii) Extension: thermostable Taq DNA polymerase extends the primers using the nucleotides provided.

∴ Correct order: denaturation, annealing, extension.

Answer: (4)

NEET 2018

Keep practising — Biotechnology: Principles and Processes

2

The DNA fragments separated on an agarose gel can be visualised after staining with

  1. Bromophenol blue
  2. Aniline blue
  3. Ethidium bromide
  4. Acetocarmine
NEET 2017
3

DNA fragments are

  1. Positively charged
  2. Either positively or negatively charged depending on their size
  3. Neutral
  4. Negatively charged
NEET 2017
4

A gene whose expression helps to identify transformed cell is known as

  1. Selectable marker
  2. Plasmid
  3. Vector
  4. Structural gene
NEET 2017
5

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

  1. Positively charged fragments move to farther end
  2. Negatively charged fragments do not move
  3. The smaller the fragment size, the farther it moves
  4. The larger the fragment size, the farther it moves
NEET 2017
6

The process of separation and purification of expressed protein before marketing is called :

  1. Upstream processing
  2. Bioprocessing
  3. Downstream processing
  4. Postproduction processing
NEET 2017
7

Which of the following is not a feature of the plasmids ?

  1. Single-stranded
  2. Transferable
  3. Independent replication
  4. Circular structure
NEET 2016 (Phase 1)
8

The taq polymerase enzyme is obtained from

  1. Thermus aquaticus
  2. Bacillus subtilis
  3. Pseudomonas putida
  4. Thiobacillus ferrooxidans
NEET 2016 (Phase 1)
9

Which of the following is a restriction endonuclease ?

  1. DNase I
  2. Hind II
  3. RNase I
  4. Protease
NEET 2016 (Phase 1)
10

Stirred-tank bioreactors have been designed for -

  1. ensuring anaerobic conditions in the culture vessel
  2. addition of preservatives to the product
  3. purification of product
  4. availability of oxygen throughout the process
NEET 2016 (Phase 2)
11

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

  1. Eco RI
  2. Polymerase III
  3. Ligase
  4. Taq polymerase
NEET 2016 (Phase 2)

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