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Biotechnology: Principles and Processes · Question 61

NEET 2017MCQEasy📈 Topic in 88% papers+4−1
1

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

  1. Positively charged fragments move to farther end
  2. Negatively charged fragments do not move
  3. The smaller the fragment size, the farther it moves
  4. The larger the fragment size, the farther it moves

Answer

(3) The smaller the fragment size, the farther it moves

Solution

DNA fragments are negatively charged, all of them move towards the anode.

As per NCERT, the fragments separate according to their size through the sieving effect of the agarose gel.

The smaller the fragment size, the farther it moves.

Answer: (3)

NEET 2017

Keep practising — Biotechnology: Principles and Processes

2

The process of separation and purification of expressed protein before marketing is called :

  1. Upstream processing
  2. Bioprocessing
  3. Downstream processing
  4. Postproduction processing
NEET 2017
3

Which of the following is not a feature of the plasmids ?

  1. Single-stranded
  2. Transferable
  3. Independent replication
  4. Circular structure
NEET 2016 (Phase 1)
4

The taq polymerase enzyme is obtained from

  1. Thermus aquaticus
  2. Bacillus subtilis
  3. Pseudomonas putida
  4. Thiobacillus ferrooxidans
NEET 2016 (Phase 1)
5

Which of the following is a restriction endonuclease ?

  1. DNase I
  2. Hind II
  3. RNase I
  4. Protease
NEET 2016 (Phase 1)
6

Stirred-tank bioreactors have been designed for -

  1. ensuring anaerobic conditions in the culture vessel
  2. addition of preservatives to the product
  3. purification of product
  4. availability of oxygen throughout the process
NEET 2016 (Phase 2)
7

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

  1. Eco RI
  2. Polymerase III
  3. Ligase
  4. Taq polymerase
NEET 2016 (Phase 2)
8

Which of the following is not a component of downstream processing ?

  1. Purification
  2. Expression
  3. Preservation
  4. Separation
NEET 2016 (Phase 2)
9

Which of the following restriction enzymes produces blunt ends ?

  1. Eco RV
  2. Hind III
  3. Xho I
  4. Sal I
NEET 2016 (Phase 2)
10

The DNA molecule to which the gene of interest is integrated for cloning is called -

  1. Transformer
  2. Template
  3. Vector
  4. Carrier
AIPMT 2015
11

The cutting of DNA at specific locations became possible with the discovery of -

  1. Restriction enzymes
  2. Selectable markers
  3. Probes
  4. Ligases
AIPMT 2015

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