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Biotechnology and its Applications · Question 64

AIPMT 2009MCQEasy📈 Topic in 75% papers+4−1
1

What is antisense technology?

  1. RNA polymerase producing DNA
  2. Production of somaclonal variants in tissue cultures
  3. When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
  4. A cell displaying a foreign antigen used for synthesis of antigens

Answer

(3) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

Solution

As per NCERT, RNA interference (RNAi) silences a specific mRNA by a complementary dsRNA molecule that binds to it and prevents its translation.

In antisense technology, an RNA strand complementary (antisense) to the mRNA of a gene is introduced.

It pairs with the mRNA, so the mRNA cannot be translated and the expression of that specific gene is stopped.

Answer: (3)

AIPMT 2009

Keep practising — Biotechnology and its Applications

2

Cry I endotoxins obtained from Bacillus thuringiensis are effective against:

  1. Mosquitoes
  2. Flies
  3. Nematodes
  4. Boll worms
AIPMT 2008
3

Main objective of production/use of herbicide resistant GM crops is to:

  1. eliminate weeds from the field without the use of manual labour
  2. eliminate weeds from the field without the use of herbicides
  3. encourage eco-friendly herbicides
  4. reduce herbicide accumulation in food articles for health safety
AIPMT 2008
4

Human insulin is being commercially produced from a transgenic species of:

  1. Escherichia
  2. Mycobacterium
  3. Rhizobium
  4. Saccharomyces
AIPMT 2008
5

A transgenic food crop which may help in solving the problem of night blindness in developing countries is

  1. Flavr Savr tomatoes
  2. Bt soybean
  3. Starlink maize
  4. Golden rice
AIPMT 2008
6

Golden rice is a promising transgenic crop. When released for cultivation, it will help in

  1. Herbicide tolerance
  2. Alleviation of vitamin A deficiency
  3. Producing a petrol-like fuel from rice
  4. Pest resistance
AIPMT 2006
7

Bacillus thuringiensis (Bt) strains have been used for designing novel -

  1. Bio-metallurgical techniques
  2. Biofertilizers
  3. Bioinsecticidal plants
  4. Bio-mineralization processes
AIPMT 2005
8

Golden rice is a transgenic crop of the future with the following improved trait -

  1. High lysine (essential amino acid) content
  2. High protein content
  3. Insect resistance
  4. High vitamin A content
AIPMT 2005
9

ELISA is used to detect viruses, where the key reagent is :-

  1. Catalase
  2. RNase
  3. DNA probes
  4. Alkaline phosphatase
AIPMT 2004
10

Maximum application of animal cell culture technology today is in the production of :-

  1. Interferons
  2. Vaccines
  3. Edible proteins
  4. Insulin
AIPMT 2003
11

ELISA is used to detect viruses, where the key reagent is :-

  1. Alkaline phosphatase
  2. RNase
  3. DNA probes
  4. Catalase
AIPMT 2003

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