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Biotechnology: Principles and Processes · Question 112

AIPMT 2001MCQEasy📈 Topic in 88% papers+4−1
1

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase

Answer

(3) E. coli restriction endonuclease I

Solution

As per NCERT, restriction endonucleases make cuts at specific positions within the DNA; each recognises a specific palindromic nucleotide sequence.

∵ EcoRI (from E. coli RY 13) cuts DNA between G and A at the sequence GAATTC.

⇒ Exonucleases remove nucleotides only from the ends of DNA, alkaline phosphatase removes terminal phosphate groups and ligase joins DNA fragments.

∴ E. coli restriction endonuclease I cuts DNA at specific places.

Answer: (3)

AIPMT 2001

Keep practising — Biotechnology: Principles and Processes

2

Maximum number of bases in plasmids discovered so far:

  1. 500 kilobases
  2. 5 kilobases
  3. 50 kilobases
  4. 5000 kilobases
AIPMT 2001
3

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus
AIPMT 2000
4

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells
AIPMT 2000

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