1
Manipulation of DNA in genetic engineering became possible due to the discovery of : -
- Transcriptase
- DNA ligase
- Primase
- Restriction endonuclease
Answer
(4) Restriction endonuclease
Solution
As per NCERT, the cutting of DNA at specific locations became possible with the discovery of the 'molecular scissors' — restriction enzymes (restriction endonucleases).
⇒ Each restriction endonuclease recognises a specific palindromic sequence and cuts DNA there, so a desired gene can be cut out and inserted into a vector.
∴ Manipulation of DNA in genetic engineering became possible due to the discovery of restriction endonuclease.
Answer: (4)
AIPMT 2002