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Biotechnology and its Applications · Question 51

AIPMT 2011 (Mains)MCQMedium📈 Topic in 75% papers+4−1
1

Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein:

  1. is activated by acid pH of the foregut of the insect pest.
  2. binds with epithelial cells of midgut of the insect pest ultimately killing it
  3. is coded by several genes including the gene cry
  4. does not kill the carrier bacterium which is itself resistant to this toxin

Answer

(2) binds with epithelial cells of midgut of the insect pest ultimately killing it

Solution

As per NCERT, the Bt toxin protein exists as an inactive protoxin; once an insect ingests it, the inactive toxin is converted into the active form due to the alkaline pH of the gut, which solubilises the crystals.

⇒ The activated toxin binds to the surface of midgut epithelial cells and creates pores that cause cell swelling and lysis, and eventually the death of the insect.

(1) is wrong as the pH is alkaline, not acidic. (4) is wrong as the bacterium is not killed because the toxin exists inside it as an inactive protoxin, not because the bacterium is resistant.

Answer: (2)

AIPMT 2011 (Mains)

Keep practising — Biotechnology and its Applications

2

Silencing of mRNA has been used in producing transgenic plants resistant to:

  1. White rusts
  2. Nematodes
  3. Bacterial blights
  4. Bollworms
AIPMT 2011 (Mains)
3

Maximum number of existing transgenic animals is of :

  1. Mice
  2. Cow
  3. Pig
  4. Fish
AIPMT 2011 (Prelims)
4

The process of RNA interference has been used in the development of plants resistant to :

  1. Nematodes
  2. Fungi
  3. Viruses
  4. Insects
AIPMT 2011 (Prelims)
5

Which one of the following is now being commercially produced by biotechnological procedures?

  1. Insulin
  2. Morphine
  3. Nicotine
  4. Quinine
AIPMT 2010 (Mains)
6

DNA or RNA segment tagged with a radioactive molecule is called –

  1. Clone
  2. Probe
  3. Plasmid
  4. Vector
AIPMT 2010 (Prelims)
7

The genetically-modified (GM) brinjal in India has been developed for:

  1. Enhancing shelf life
  2. Insect-resistance
  3. Drought-resistance
  4. Enhancing mineral content
AIPMT 2010 (Prelims)
8

Genetic engineering has been successfully used for producing –

  1. transgenic models for studying new treatments for certain cardiac disease
  2. animals like bulls for farm work as they have super power
  3. transgenic mice for testing safety of polio vaccine before use in humans
  4. transgenic Cow-Rosie which produces high fat milk for making ghee
AIPMT 2010 (Prelims)
9

Some of the characteristics of Bt cotton are –

  1. High yield and production of toxic protein crystals which kill dipteran pests
  2. High yield and resistance to bollworms
  3. Long fibre and resistance to aphids
  4. Medium yield, long fibre and resistance to beetle pests
AIPMT 2010 (Prelims)
10

An improved variety of transgenic basmati rice

  1. gives high yield but has no characteristic aroma
  2. does not require chemical fertilizers and growth hormones
  3. is completely resistant to all insect pests and diseases of paddy
  4. gives high yield and is rich in vitamin A
AIPMT 2010 (Prelims)
11

The genetic defect – adenosine deaminase (ADA) deficiency may be cured permanently by

  1. Introducing bone marrow cells producing ADA into cells at early embryonic stages
  2. Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA
  3. Administering adenosine deaminase activators
  4. Enzyme replacement therapy
AIPMT 2009

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