Biotechnology: Principles and Processes · Question 83

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cell
2. It remains active at high temperature
3. It serves as a selectable marker
4. It is isolated from a virus

For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of

1. Gold or tungsten
2. Platinum or zinc
3. Silver or platinum
4. Silicon or platinum

Which one of the following techniques made it possible to genetically engineer living organisms?

1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization

Read the following four statements (A-D) with certain mistakes in two of them. Which are the two statements having mistakes?

1. Statements (B) and (C)
2. Statements (A) and (C)
3. Statements (A) and (B)
4. Statements (C) and (D)

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it ? 5' .............. GAATTC ................ 3' 3' .............. CTTAAG ................ 5'

1. Start codon at the 5' end
2. Replication completed
3. Deletion mutation
4. Palindromic sequence of base pairs

There is a restriction endonuclease called EcoRI. What does "co" part in it stand for ?

1. colon
2. coelom
3. coenzyme
4. coli

Agarose extracted from sea weeds finds use in :

1. Spectrophotometry
2. PCR
3. Tissue Culture
4. Gel electrophoresis

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector / vectors –

1. B and D only
2. A and C only
3. A, B and D only
4. A only

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

1. 5′ —— CGTTCG —— 3′ 3′ —— ATGGTA —— 5′
2. 5′ —— GAATTC —— 3′ 3′ —— CTTAAG —— 5′
3. 5′ —— CACGTA —— 3′ 3′ —— CTCAGT —— 5′
4. 5′ —— GATATG —— 3′ 3′ —— CTACTA —— 5′

Which one of the following is used as vector for cloning genes into higher organisms ?

1. Retrovirus
2. Salmonella typhimurium
3. Rhizopus nigricans
4. Baculovirus