Biotechnology: Principles and Processes · Question 87

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it ? 5' .............. GAATTC ................ 3' 3' .............. CTTAAG ................ 5'

1. Start codon at the 5' end
2. Replication completed
3. Deletion mutation
4. Palindromic sequence of base pairs

There is a restriction endonuclease called EcoRI. What does "co" part in it stand for ?

1. colon
2. coelom
3. coenzyme
4. coli

Agarose extracted from sea weeds finds use in :

1. Spectrophotometry
2. PCR
3. Tissue Culture
4. Gel electrophoresis

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector / vectors –

1. B and D only
2. A and C only
3. A, B and D only
4. A only

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

1. 5′ —— CGTTCG —— 3′ 3′ —— ATGGTA —— 5′
2. 5′ —— GAATTC —— 3′ 3′ —— CTTAAG —— 5′
3. 5′ —— CACGTA —— 3′ 3′ —— CTCAGT —— 5′
4. 5′ —— GATATG —— 3′ 3′ —— CTACTA —— 5′

Which one of the following is used as vector for cloning genes into higher organisms ?

1. Retrovirus
2. Salmonella typhimurium
3. Rhizopus nigricans
4. Baculovirus

Restriction endonucleases are enzymes which –

1. recognize a specific nucleotide sequence for binding of DNA ligase
2. remove nucleotides from the ends of the DNA molecule
3. restrict the action of the enzyme DNA polymerase
4. make cuts at specific positions within the DNA molecule

Stirred-tank bioreactors have been designed for –

1. Purification of the product
2. Availability of oxygen throughout the process
3. Ensuring anaerobic conditions in the culture vessel
4. Addition of preservatives to the product

Which of the following are used in gene cloning ?

1. Mesosomes
2. Nucleoids
3. Lomasomes
4. Plasmids

Polyethylene glycol method is used for:

1. Biodiesel production
2. Seedless fruit production
3. Energy production from sewage
4. Gene transfer without a vector