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Biotechnology: Principles and Processes · Question 88

AIPMT 2011 (Prelims)MCQEasy📈 Topic in 88% papers+4−1
1

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it ? 5' .............. GAATTC ................ 3' 3' .............. CTTAAG ................ 5'

  1. Start codon at the 5' end
  2. Replication completed
  3. Deletion mutation
  4. Palindromic sequence of base pairs

Answer

(4) Palindromic sequence of base pairs

Solution

Reading both strands in the 5' 3' direction:

upper strand ⇒ GAATTC

lower strand ⇒ GAATTC

∵ The sequence reads the same on both strands when the orientation of reading is kept the same,

∴ as per NCERT, it is a palindromic sequence of base pairs (the recognition site of EcoRI).

Answer: (4)

AIPMT 2011 (Prelims)

Keep practising — Biotechnology: Principles and Processes

2

There is a restriction endonuclease called EcoRI. What does "co" part in it stand for ?

  1. colon
  2. coelom
  3. coenzyme
  4. coli
AIPMT 2011 (Prelims)
3

Agarose extracted from sea weeds finds use in :

  1. Spectrophotometry
  2. PCR
  3. Tissue Culture
  4. Gel electrophoresis
AIPMT 2011 (Prelims)
4

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector / vectors –

  1. B and D only
  2. A and C only
  3. A, B and D only
  4. A only
AIPMT 2010 (Mains)
5

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

  1. 5′ —— CGTTCG —— 3′ 3′ —— ATGGTA —— 5′
  2. 5′ —— GAATTC —— 3′ 3′ —— CTTAAG —— 5′
  3. 5′ —— CACGTA —— 3′ 3′ —— CTCAGT —— 5′
  4. 5′ —— GATATG —— 3′ 3′ —— CTACTA —— 5′
AIPMT 2010 (Prelims)
6

Which one of the following is used as vector for cloning genes into higher organisms ?

  1. Retrovirus
  2. Salmonella typhimurium
  3. Rhizopus nigricans
  4. Baculovirus
AIPMT 2010 (Prelims)
7

Restriction endonucleases are enzymes which –

  1. recognize a specific nucleotide sequence for binding of DNA ligase
  2. remove nucleotides from the ends of the DNA molecule
  3. restrict the action of the enzyme DNA polymerase
  4. make cuts at specific positions within the DNA molecule
AIPMT 2010 (Prelims)
8

Stirred-tank bioreactors have been designed for –

  1. Purification of the product
  2. Availability of oxygen throughout the process
  3. Ensuring anaerobic conditions in the culture vessel
  4. Addition of preservatives to the product
AIPMT 2010 (Prelims)
9

Which of the following are used in gene cloning ?

  1. Mesosomes
  2. Nucleoids
  3. Lomasomes
  4. Plasmids
AIPMT 2010 (Prelims)
10

Polyethylene glycol method is used for:

  1. Biodiesel production
  2. Seedless fruit production
  3. Energy production from sewage
  4. Gene transfer without a vector
AIPMT 2009
11

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  1. Trichoderma harzianum
  2. Meloidogyne incognita
  3. Agrobacterium tumefaciens
  4. Penicillium expansum
AIPMT 2009

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