Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
- Retrovirus
- Ti plasmid
- phage
- pBR322
Answer
(1) Retrovirus
Solution
As per NCERT, in gene therapy for ADA deficiency, lymphocytes from the blood of the patient are grown in culture, and a functional ADA cDNA is introduced into these lymphocytes using a retroviral vector; the lymphocytes are then returned to the patient.
Ti plasmid is a vector for plant cells, and phage and pBR322 are vectors for bacteria ( E. coli).
∴ Retrovirus is used as the vector for human lymphocytes.
Answer: (1)