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Biotechnology: Principles and Processes · Question 55

NEET (UG) 2019MCQEasy📈 Topic in 88% papers+4−1
1

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

  1. The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
  2. The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
  3. The enzyme cuts DNA molecule at identified position within the DNA
  4. The enzyme binds DNA at specific sites and cuts only one of the two strands

Answer

(4) The enzyme binds DNA at specific sites and cuts only one of the two strands

Solution

As per NCERT, each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA, binds to it and cuts each of the two strands of the double helix at specific points in their sugar-phosphate backbones.

∴ Statements (1), (2) and (3) are correct.

∵ The enzyme cuts both strands (not only one), statement (4) is incorrect.

Answer: (4)

NEET (UG) 2019

Keep practising — Biotechnology: Principles and Processes

2

Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

  1. Industrial oven
  2. Bioreactor
  3. BOD incubator
  4. Sludge digester
Out of syllabusNEET (UG) 2019
3

The correct order of steps in Polymerase Chain Reaction (PCR) is

  1. extension, denaturation, annealing
  2. annealing, extension, denaturation
  3. denaturation, extension, annealing
  4. denaturation, annealing, extension
NEET 2018
4

The DNA fragments separated on an agarose gel can be visualised after staining with

  1. Bromophenol blue
  2. Aniline blue
  3. Ethidium bromide
  4. Acetocarmine
NEET 2017
5

DNA fragments are

  1. Positively charged
  2. Either positively or negatively charged depending on their size
  3. Neutral
  4. Negatively charged
NEET 2017
6

A gene whose expression helps to identify transformed cell is known as

  1. Selectable marker
  2. Plasmid
  3. Vector
  4. Structural gene
NEET 2017
7

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

  1. Positively charged fragments move to farther end
  2. Negatively charged fragments do not move
  3. The smaller the fragment size, the farther it moves
  4. The larger the fragment size, the farther it moves
NEET 2017
8

The process of separation and purification of expressed protein before marketing is called :

  1. Upstream processing
  2. Bioprocessing
  3. Downstream processing
  4. Postproduction processing
NEET 2017
9

Which of the following is not a feature of the plasmids ?

  1. Single-stranded
  2. Transferable
  3. Independent replication
  4. Circular structure
NEET 2016 (Phase 1)
10

The taq polymerase enzyme is obtained from

  1. Thermus aquaticus
  2. Bacillus subtilis
  3. Pseudomonas putida
  4. Thiobacillus ferrooxidans
NEET 2016 (Phase 1)
11

Which of the following is a restriction endonuclease ?

  1. DNase I
  2. Hind II
  3. RNase I
  4. Protease
NEET 2016 (Phase 1)

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