For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
- Hybridization Technique
- Western Blotting Technique
- Southern Blotting Technique
- ELISA Technique
Answer
(4) ELISA Technique
Solution
As per NCERT, using conventional methods early detection is not possible; recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis.
ELISA is based on the principle of antigen–antibody interaction; infection by a pathogen can be detected by the presence of antigens (proteins, glycoproteins etc.) or by detecting the antibodies synthesised against the pathogen.
∴ ELISA technique is very useful for early detection.
Answer: (4)