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Biotechnology: Principles and Processes · Question 93

AIPMT 2010 (Prelims)MCQEasy📈 Topic in 88% papers+4−1
1

Which one of the following is used as vector for cloning genes into higher organisms ?

  1. Retrovirus
  2. Salmonella typhimurium
  3. Rhizopus nigricans
  4. Baculovirus

Answer

(1) Retrovirus

Solution

As per NCERT, retroviruses in animals have the ability to transform normal cells into cancerous cells; disarmed retroviruses are now used to deliver desirable genes into animal cells.

Retrovirus is used as a vector for cloning genes into higher organisms (animals).

Salmonella typhimurium is a bacterium (its plasmid was used in the first rDNA), Rhizopus nigricans is a fungus, and baculoviruses are used as biocontrol agents of insects.

Answer: (1)

AIPMT 2010 (Prelims)

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2

Restriction endonucleases are enzymes which –

  1. recognize a specific nucleotide sequence for binding of DNA ligase
  2. remove nucleotides from the ends of the DNA molecule
  3. restrict the action of the enzyme DNA polymerase
  4. make cuts at specific positions within the DNA molecule
AIPMT 2010 (Prelims)
3

Stirred-tank bioreactors have been designed for –

  1. Purification of the product
  2. Availability of oxygen throughout the process
  3. Ensuring anaerobic conditions in the culture vessel
  4. Addition of preservatives to the product
AIPMT 2010 (Prelims)
4

Which of the following are used in gene cloning ?

  1. Mesosomes
  2. Nucleoids
  3. Lomasomes
  4. Plasmids
AIPMT 2010 (Prelims)
5

Polyethylene glycol method is used for:

  1. Biodiesel production
  2. Seedless fruit production
  3. Energy production from sewage
  4. Gene transfer without a vector
AIPMT 2009
6

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  1. Trichoderma harzianum
  2. Meloidogyne incognita
  3. Agrobacterium tumefaciens
  4. Penicillium expansum
AIPMT 2009
7

Gel electrophoresis is used for:

  1. cutting of DNA into fragments
  2. separation of DNA fragments according to their size
  3. construction of recombinant DNA by joining with cloning vectors
  4. isolation of DNA molecule
AIPMT 2008
8

The linking of antibiotic resistance gene with the plasmid vector became possible with:

  1. Exonucleases
  2. Endonucleases
  3. DNA ligase
  4. DNA polymerase
AIPMT 2008
9

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

  1. Trichoderma
  2. Xanthomonas
  3. Bacillus
  4. Pseudomonas
AIPMT 2007
10

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly
AIPMT 2006
11

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
AIPMT 2006

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