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Biotechnology: Principles and Processes · Question 94

AIPMT 2010 (Prelims)MCQEasy📈 Topic in 88% papers+4−1
1

Restriction endonucleases are enzymes which –

  1. recognize a specific nucleotide sequence for binding of DNA ligase
  2. remove nucleotides from the ends of the DNA molecule
  3. restrict the action of the enzyme DNA polymerase
  4. make cuts at specific positions within the DNA molecule

Answer

(4) make cuts at specific positions within the DNA molecule

Solution

As per NCERT, each restriction endonuclease inspects the length of a DNA sequence; once it finds its specific recognition sequence (palindrome), it binds to the DNA and cuts each of the two strands at specific points in the sugar-phosphate backbone.

Endonucleases make cuts at specific positions within the DNA, whereas exonucleases remove nucleotides from the ends of DNA.

Answer: (4)

AIPMT 2010 (Prelims)

Keep practising — Biotechnology: Principles and Processes

2

Stirred-tank bioreactors have been designed for –

  1. Purification of the product
  2. Availability of oxygen throughout the process
  3. Ensuring anaerobic conditions in the culture vessel
  4. Addition of preservatives to the product
AIPMT 2010 (Prelims)
3

Which of the following are used in gene cloning ?

  1. Mesosomes
  2. Nucleoids
  3. Lomasomes
  4. Plasmids
AIPMT 2010 (Prelims)
4

Polyethylene glycol method is used for:

  1. Biodiesel production
  2. Seedless fruit production
  3. Energy production from sewage
  4. Gene transfer without a vector
AIPMT 2009
5

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  1. Trichoderma harzianum
  2. Meloidogyne incognita
  3. Agrobacterium tumefaciens
  4. Penicillium expansum
AIPMT 2009
6

Gel electrophoresis is used for:

  1. cutting of DNA into fragments
  2. separation of DNA fragments according to their size
  3. construction of recombinant DNA by joining with cloning vectors
  4. isolation of DNA molecule
AIPMT 2008
7

The linking of antibiotic resistance gene with the plasmid vector became possible with:

  1. Exonucleases
  2. Endonucleases
  3. DNA ligase
  4. DNA polymerase
AIPMT 2008
8

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

  1. Trichoderma
  2. Xanthomonas
  3. Bacillus
  4. Pseudomonas
AIPMT 2007
9

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly
AIPMT 2006
10

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
AIPMT 2006
11

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

  1. Protease
  2. Endonuclease
  3. Lipase
  4. Exonuclease
AIPMT 2005

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