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Biotechnology: Principles and Processes · Question 96

AIPMT 2010 (Prelims)MCQEasy📈 Topic in 88% papers+4−1
1

Which of the following are used in gene cloning ?

  1. Mesosomes
  2. Nucleoids
  3. Lomasomes
  4. Plasmids

Answer

(4) Plasmids

Solution

As per NCERT, plasmids are autonomously replicating circular extra-chromosomal DNA of bacteria; they have an origin of replication and are used as vectors to carry and multiply the gene of interest in gene cloning (e.g. pBR322).

Mesosomes and nucleoids are cell structures of bacteria, and lomasomes are membranous bodies of fungal cells; they are not used in cloning.

Answer: (4)

AIPMT 2010 (Prelims)

Keep practising — Biotechnology: Principles and Processes

2

Polyethylene glycol method is used for:

  1. Biodiesel production
  2. Seedless fruit production
  3. Energy production from sewage
  4. Gene transfer without a vector
AIPMT 2009
3

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  1. Trichoderma harzianum
  2. Meloidogyne incognita
  3. Agrobacterium tumefaciens
  4. Penicillium expansum
AIPMT 2009
4

Gel electrophoresis is used for:

  1. cutting of DNA into fragments
  2. separation of DNA fragments according to their size
  3. construction of recombinant DNA by joining with cloning vectors
  4. isolation of DNA molecule
AIPMT 2008
5

The linking of antibiotic resistance gene with the plasmid vector became possible with:

  1. Exonucleases
  2. Endonucleases
  3. DNA ligase
  4. DNA polymerase
AIPMT 2008
6

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

  1. Trichoderma
  2. Xanthomonas
  3. Bacillus
  4. Pseudomonas
AIPMT 2007
7

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly
AIPMT 2006
8

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
AIPMT 2006
9

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

  1. Protease
  2. Endonuclease
  3. Lipase
  4. Exonuclease
AIPMT 2005
10

The most thoroughly studied of the known bacteria-plant interactions is the :-

  1. Nodulation of Sesbania stems by nitrogen fixing bacteria
  2. Cyanobacterial symbiosis with some aquatic ferns
  3. Plant growth stimulation by phosphate-solubilising bacteria
  4. Gall formation on certain angiosperms by Agrobacterium
AIPMT 2004
11

Restriction endonucleases :-

  1. Are used for in vitro DNA synthesis
  2. Are present in mammalian cells for degradation of DNA when the cell dies
  3. Are synthesized by bacteria as part of their defense mechanism
  4. Are used in genetic engineering for ligating two DNA molecules
AIPMT 2004

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