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Biotechnology: Principles and Processes · Question 100

AIPMT 2008MCQEasy📈 Topic in 88% papers+4−1
1

The linking of antibiotic resistance gene with the plasmid vector became possible with:

  1. Exonucleases
  2. Endonucleases
  3. DNA ligase
  4. DNA polymerase

Answer

(3) DNA ligase

Solution

⇒ Restriction endonucleases only cut DNA at specific recognition sequences, producing sticky ends; exonucleases remove nucleotides from the ends.

⇒ The cut antibiotic resistance gene and the cut plasmid vector are joined by forming phosphodiester bonds between their sugar–phosphate backbones.

∴ As per NCERT, the enzyme DNA ligase joins the DNA fragments, so the linking of an antibiotic resistance gene with a plasmid vector (the first recombinant DNA, made by Cohen and Boyer in 1972) became possible with DNA ligase.

Answer: (3)

AIPMT 2008

Keep practising — Biotechnology: Principles and Processes

2

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

  1. Trichoderma
  2. Xanthomonas
  3. Bacillus
  4. Pseudomonas
AIPMT 2007
3

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly
AIPMT 2006
4

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
AIPMT 2006
5

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

  1. Protease
  2. Endonuclease
  3. Lipase
  4. Exonuclease
AIPMT 2005
6

The most thoroughly studied of the known bacteria-plant interactions is the :-

  1. Nodulation of Sesbania stems by nitrogen fixing bacteria
  2. Cyanobacterial symbiosis with some aquatic ferns
  3. Plant growth stimulation by phosphate-solubilising bacteria
  4. Gall formation on certain angiosperms by Agrobacterium
AIPMT 2004
7

Restriction endonucleases :-

  1. Are used for in vitro DNA synthesis
  2. Are present in mammalian cells for degradation of DNA when the cell dies
  3. Are synthesized by bacteria as part of their defense mechanism
  4. Are used in genetic engineering for ligating two DNA molecules
AIPMT 2004
8

In transgenics expression of transgene in target tissue is determined by :-

  1. Enhancer
  2. Promoter
  3. Reporter
  4. Transgene
AIPMT 2004
9

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

  1. Agrobacterium
  2. Rhizobium of the roots of leguminous plants
  3. Azotobacter
  4. Yeast as a 2 plasmid
AIPMT 2004
10

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

  1. Xanthomonas citri
  2. Agrobacterium tumefaciens
  3. Bacillus coagulans
  4. Clostridium septicum
AIPMT 2003
11

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase
AIPMT 2002

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