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Biotechnology: Principles and Processes · Question 98

AIPMT 2009MCQEasy📈 Topic in 88% papers+4−1
1

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  1. Trichoderma harzianum
  2. Meloidogyne incognita
  3. Agrobacterium tumefaciens
  4. Penicillium expansum

Answer

(3) Agrobacterium tumefaciens

Solution

As per NCERT, Agrobacterium tumefaciens, a pathogen of several dicot plants, delivers a piece of DNA (T-DNA) into plant cells.

Its tumour-inducing (Ti) plasmid has been modified into a cloning vector that is no longer pathogenic but still delivers genes of interest into plants.

Agrobacterium tumefaciens is used to transfer foreign DNA into crop plants.

Answer: (3)

AIPMT 2009

Keep practising — Biotechnology: Principles and Processes

2

Gel electrophoresis is used for:

  1. cutting of DNA into fragments
  2. separation of DNA fragments according to their size
  3. construction of recombinant DNA by joining with cloning vectors
  4. isolation of DNA molecule
AIPMT 2008
3

The linking of antibiotic resistance gene with the plasmid vector became possible with:

  1. Exonucleases
  2. Endonucleases
  3. DNA ligase
  4. DNA polymerase
AIPMT 2008
4

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

  1. Trichoderma
  2. Xanthomonas
  3. Bacillus
  4. Pseudomonas
AIPMT 2007
5

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly
AIPMT 2006
6

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
AIPMT 2006
7

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

  1. Protease
  2. Endonuclease
  3. Lipase
  4. Exonuclease
AIPMT 2005
8

The most thoroughly studied of the known bacteria-plant interactions is the :-

  1. Nodulation of Sesbania stems by nitrogen fixing bacteria
  2. Cyanobacterial symbiosis with some aquatic ferns
  3. Plant growth stimulation by phosphate-solubilising bacteria
  4. Gall formation on certain angiosperms by Agrobacterium
AIPMT 2004
9

Restriction endonucleases :-

  1. Are used for in vitro DNA synthesis
  2. Are present in mammalian cells for degradation of DNA when the cell dies
  3. Are synthesized by bacteria as part of their defense mechanism
  4. Are used in genetic engineering for ligating two DNA molecules
AIPMT 2004
10

In transgenics expression of transgene in target tissue is determined by :-

  1. Enhancer
  2. Promoter
  3. Reporter
  4. Transgene
AIPMT 2004
11

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

  1. Agrobacterium
  2. Rhizobium of the roots of leguminous plants
  3. Azotobacter
  4. Yeast as a 2 plasmid
AIPMT 2004

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