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Biotechnology: Principles and Processes · Question 102

AIPMT 2006MCQEasy📈 Topic in 88% papers+4−1
1

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly

Answer

(1) Cuts the DNA molecule at specific sites

Solution

As per NCERT, each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA.

⇒ It binds to the DNA and cuts each of the two strands at specific points in their sugar–phosphate backbones.

⇒ E.g. EcoRI cuts between G and A in the sequence 5′-GAATTC-3′.

∴ Restriction endonucleases cut DNA at specific sites, not randomly.

Answer: (1)

AIPMT 2006

Keep practising — Biotechnology: Principles and Processes

2

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
AIPMT 2006
3

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

  1. Protease
  2. Endonuclease
  3. Lipase
  4. Exonuclease
AIPMT 2005
4

The most thoroughly studied of the known bacteria-plant interactions is the :-

  1. Nodulation of Sesbania stems by nitrogen fixing bacteria
  2. Cyanobacterial symbiosis with some aquatic ferns
  3. Plant growth stimulation by phosphate-solubilising bacteria
  4. Gall formation on certain angiosperms by Agrobacterium
AIPMT 2004
5

Restriction endonucleases :-

  1. Are used for in vitro DNA synthesis
  2. Are present in mammalian cells for degradation of DNA when the cell dies
  3. Are synthesized by bacteria as part of their defense mechanism
  4. Are used in genetic engineering for ligating two DNA molecules
AIPMT 2004
6

In transgenics expression of transgene in target tissue is determined by :-

  1. Enhancer
  2. Promoter
  3. Reporter
  4. Transgene
AIPMT 2004
7

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

  1. Agrobacterium
  2. Rhizobium of the roots of leguminous plants
  3. Azotobacter
  4. Yeast as a 2 plasmid
AIPMT 2004
8

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

  1. Xanthomonas citri
  2. Agrobacterium tumefaciens
  3. Bacillus coagulans
  4. Clostridium septicum
AIPMT 2003
9

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase
AIPMT 2002
10

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

  1. Transcriptase
  2. DNA ligase
  3. Primase
  4. Restriction endonuclease
AIPMT 2002
11

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase
AIPMT 2001

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