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Biotechnology and its Applications · Question 46

NEET 2013 (Karnataka)MCQMedium📈 Topic in 12% papers+4−1
1

Which one of the following vectors is used to replace the defective gene in gene therapy ?

  1. Ti plasmid
  2. Cosmid
  3. Ri plasmid
  4. Adenovirus

Answer

(4) Adenovirus

Solution

In gene therapy a normal (functional) gene is delivered into the cells of a person to correct a defective gene; this needs a vector that can infect human cells.

⇒ Disabled animal viruses such as adenoviruses and retroviruses are used as vectors for this purpose. (As per NCERT, in the first gene therapy for ADA deficiency, functional ADA cDNA was introduced into lymphocytes using a retroviral vector.)

∵ Ti and Ri plasmids (of Agrobacterium) are vectors for plant cells, and a cosmid is a cloning vector for large DNA fragments in bacteria, they are not used in human gene therapy.

∴ Among the options, adenovirus is the vector used in gene therapy.

Answer: (4)

NEET 2013 (Karnataka)

Keep practising — Biotechnology and its Applications

2

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) :

  1. An antifeedant
  2. A toxic protein
  3. Both sense and anti-sense RNA
  4. A particular hormone
AIPMT 2012 (Mains)
3

The first clinical gene therapy was given for treating

  1. Rheumatoid arthritis
  2. Adenosine deaminase deficiency
  3. Diabetes mellitus
  4. Chicken pox
AIPMT 2012 (Mains)
4

A single strand of nucleic acid tagged with a radioactive molecule is called

  1. Probe
  2. Selectable marker
  3. Vector
  4. Plasmid
AIPMT 2012 (Prelims)
5

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?

  1. Flavr Savr tomato
  2. Canola
  3. Bt-brinjal
  4. Golden rice
AIPMT 2012 (Prelims)
6

Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein:

  1. is activated by acid pH of the foregut of the insect pest.
  2. binds with epithelial cells of midgut of the insect pest ultimately killing it
  3. is coded by several genes including the gene cry
  4. does not kill the carrier bacterium which is itself resistant to this toxin
AIPMT 2011 (Mains)
7

Silencing of mRNA has been used in producing transgenic plants resistant to:

  1. White rusts
  2. Nematodes
  3. Bacterial blights
  4. Bollworms
AIPMT 2011 (Mains)
8

Maximum number of existing transgenic animals is of :

  1. Mice
  2. Cow
  3. Pig
  4. Fish
AIPMT 2011 (Prelims)
9

The process of RNA interference has been used in the development of plants resistant to :

  1. Nematodes
  2. Fungi
  3. Viruses
  4. Insects
AIPMT 2011 (Prelims)
10

Which one of the following is now being commercially produced by biotechnological procedures?

  1. Insulin
  2. Morphine
  3. Nicotine
  4. Quinine
AIPMT 2010 (Mains)
11

DNA or RNA segment tagged with a radioactive molecule is called –

  1. Clone
  2. Probe
  3. Plasmid
  4. Vector
AIPMT 2010 (Prelims)

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