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Biotechnology: Principles and Processes · Question 110

AIPMT 2002MCQEasy📈 Topic in 88% papers+4−1
1

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase

Answer

(3) Ligase

Solution

As per NCERT, DNA ligase joins DNA fragments by forming phosphodiester bonds between their ends (e.g., Okazaki fragments in replication and the vector–insert DNA in recombinant DNA technology).

Endonucleases cut DNA, DNA polymerase adds nucleotides, and primase makes RNA primers.

∴ Ligase is used to join bits of DNA ('molecular glue').

Answer: (3)

AIPMT 2002

Keep practising — Biotechnology: Principles and Processes

2

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

  1. Transcriptase
  2. DNA ligase
  3. Primase
  4. Restriction endonuclease
AIPMT 2002
3

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase
AIPMT 2001
4

Maximum number of bases in plasmids discovered so far:

  1. 500 kilobases
  2. 5 kilobases
  3. 50 kilobases
  4. 5000 kilobases
AIPMT 2001
5

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus
AIPMT 2000
6

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells
AIPMT 2000

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