Biotechnology and its Applications

The genetic defect – adenosine deaminase (ADA) deficiency may be cured permanently by

1. Introducing bone marrow cells producing ADA into cells at early embryonic stages
2. Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA
3. Administering adenosine deaminase activators
4. Enzyme replacement therapy

What is true about Bt toxin?

1. The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.
2. Bt protein exists as active toxin in the Bacillus
3. The concerned Bacillus has antitoxins
4. The inactive protoxin gets converted into active form in the insect gut

Transgenic plants are the ones -

1. Produced after protoplast fusion in artificial medium
2. Produced by a somatic embryo in artificial medium
3. Generated by introducing foreign DNA into a cell and regenerating a plant from that cell
4. Grown in artificial medium after hybridization in the field

What is antisense technology?

1. RNA polymerase producing DNA
2. Production of somaclonal variants in tissue cultures
3. When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
4. A cell displaying a foreign antigen used for synthesis of antigens

Cry I endotoxins obtained from Bacillus thuringiensis are effective against:

1. Mosquitoes
2. Flies
3. Nematodes
4. Boll worms

Main objective of production/use of herbicide resistant GM crops is to:

1. eliminate weeds from the field without the use of manual labour
2. eliminate weeds from the field without the use of herbicides
3. encourage eco-friendly herbicides
4. reduce herbicide accumulation in food articles for health safety

Human insulin is being commercially produced from a transgenic species of:

1. Escherichia
2. Mycobacterium
3. Rhizobium
4. Saccharomyces

A transgenic food crop which may help in solving the problem of night blindness in developing countries is

1. Flavr Savr tomatoes
2. Bt soybean
3. Starlink maize
4. Golden rice

Golden rice is a promising transgenic crop. When released for cultivation, it will help in

1. Herbicide tolerance
2. Alleviation of vitamin A deficiency
3. Producing a petrol-like fuel from rice
4. Pest resistance

Bacillus thuringiensis (Bt) strains have been used for designing novel -

1. Bio-metallurgical techniques
2. Biofertilizers
3. Bioinsecticidal plants
4. Bio-mineralization processes

Golden rice is a transgenic crop of the future with the following improved trait -

1. High lysine (essential amino acid) content
2. High protein content
3. Insect resistance
4. High vitamin A content

ELISA is used to detect viruses, where the key reagent is :-

1. Catalase
2. RNase
3. DNA probes
4. Alkaline phosphatase

Maximum application of animal cell culture technology today is in the production of :-

1. Interferons
2. Vaccines
3. Edible proteins
4. Insulin

ELISA is used to detect viruses, where the key reagent is :-

1. Alkaline phosphatase
2. RNase
3. DNA probes
4. Catalase