PYQSide ExamsMy practice

Biotechnology: Principles and Processes · Question 107

AIPMT 2004MCQHard📈 Topic in 88% papers+4−1
1

In transgenics expression of transgene in target tissue is determined by :-

  1. Enhancer
  2. Promoter
  3. Reporter
  4. Transgene

Answer

(3) Reporter

Solution

As per NCERT, a vector needs a selectable marker to help in identifying and eliminating non-transformants.

⇒ Such marker genes, commonly called reporter genes (e.g. gus, lux, cat, nptII; β-galactosidase in insertional inactivation), are linked with the transgene.

⇒ The activity of the reporter (blue colour, luminescence, antibiotic resistance) shows whether, and in which tissue, the transgene is being expressed.

∴ Expression of the transgene in the target tissue is determined by the reporter.

Answer: (3)

AIPMT 2004

Keep practising — Biotechnology: Principles and Processes

2

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

  1. Agrobacterium
  2. Rhizobium of the roots of leguminous plants
  3. Azotobacter
  4. Yeast as a 2 plasmid
AIPMT 2004
3

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

  1. Xanthomonas citri
  2. Agrobacterium tumefaciens
  3. Bacillus coagulans
  4. Clostridium septicum
AIPMT 2003
4

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase
AIPMT 2002
5

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

  1. Transcriptase
  2. DNA ligase
  3. Primase
  4. Restriction endonuclease
AIPMT 2002
6

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase
AIPMT 2001
7

Maximum number of bases in plasmids discovered so far:

  1. 500 kilobases
  2. 5 kilobases
  3. 50 kilobases
  4. 5000 kilobases
AIPMT 2001
8

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus
AIPMT 2000
9

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells
AIPMT 2000

All Biotechnology: Principles and Processes PYQs →

PDFDownload Biotechnology: Principles and Processes PYQs as PDF Free, with answer key and a QR code to every solution — print it or study offline⬇
All NEET Zoology chapters
Class 11Animal KingdomStructural Organisation in AnimalsBiomolecules (Biology)Digestion and AbsorptionBreathing and Exchange of GasesBody Fluids and CirculationExcretory Products and their EliminationLocomotion and MovementNeural Control and CoordinationChemical Coordination and Integration
Class 12Human ReproductionReproductive HealthEvolutionHuman Health and DiseaseBiotechnology: Principles and ProcessesBiotechnology and its ApplicationsBiology Experimental Skills