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Biotechnology: Principles and Processes · Question 108

AIPMT 2004MCQEasy📈 Topic in 88% papers+4−1
1

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

  1. Agrobacterium
  2. Rhizobium of the roots of leguminous plants
  3. Azotobacter
  4. Yeast as a 2 plasmid

Answer

(1) Agrobacterium

Solution

As per NCERT, the tumour-inducing (Ti) plasmid occurs naturally in Agrobacterium tumefaciens, a pathogen of several dicot plants.

∵ It transfers a piece of its DNA (T-DNA) into the plant cell and transforms it,

∴ a disarmed (non-pathogenic) Ti plasmid is used as a cloning vector to deliver genes of interest into plants.

Answer: (1)

AIPMT 2004

Keep practising — Biotechnology: Principles and Processes

2

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

  1. Xanthomonas citri
  2. Agrobacterium tumefaciens
  3. Bacillus coagulans
  4. Clostridium septicum
AIPMT 2003
3

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase
AIPMT 2002
4

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

  1. Transcriptase
  2. DNA ligase
  3. Primase
  4. Restriction endonuclease
AIPMT 2002
5

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase
AIPMT 2001
6

Maximum number of bases in plasmids discovered so far:

  1. 500 kilobases
  2. 5 kilobases
  3. 50 kilobases
  4. 5000 kilobases
AIPMT 2001
7

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus
AIPMT 2000
8

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells
AIPMT 2000

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