Now-a-days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise to its complementary DNA in a clone of cells, followed by its detection using autoradiography because:
- mutated gene does not appear on photographic film as the probe has complementarity with it
- mutated gene partially appears on a photographic film
- mutated gene completely and clearly appears on a photographic film
- mutated gene does not appear on a photographic film as the probe has no complementarity with it
Answer
(4) mutated gene does not appear on a photographic film as the probe has no complementarity with it
Solution
As per NCERT, a single-stranded DNA or RNA tagged with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells, followed by detection using autoradiography.
The clone having the mutated gene will not appear on the photographic film, because the probe will not have complementarity with the mutated gene.
Answer: (4)