Molecular Basis of Inheritance

If a geneticist uses the blind approach for sequencing the whole genome of an organism, followed by assignment of function to different segments, the methodology adopted by him is called as :

1. Sequence annotation
2. Gene mapping
3. Expressed sequence tags
4. Bioinformatics

If the length of a DNA molecule is 1.1 metres, what will be the approximate number of base pairs?

1. 3.3 109 bp
2. 6.6 109 bp
3. 3.3 106 bp
4. 6.6 106 bp

In an E. coli strain i gene gets mutated and its product cannot bind the inducer molecule. If growth medium is provided with lactose, what will be the outcome?

1. Only z gene will get transcribed
2. z, y, a genes will be transcribed
3. z, y, a genes will not be translated
4. RNA polymerase will bind the promoter region

Ten E. coli cells with 15N-dsDNA are incubated in medium containing 14N nucleotide. After 60 minutes, how many E. coli cells will have DNA totally free from 15N?

1. 20 cells
2. 40 cells
3. 60 cells
4. 80 cells

In lac operon, z gene codes for

1. Transacetylase
2. β-galactosidase
3. Permease
4. Repressor

Given below are two statements In the light of the above statements, choose the correct answer from the options given below:

1. Statement I is incorrect but Statement II is correct
2. Both Statement I and Statement II are correct
3. Both Statement I and Statement II are incorrect
4. Statement I is correct but Statement II is incorrect

If DNA contained sulfur instead of phosphorus and proteins contained phosphorus instead of sulfur, what would have been the outcome of Hershey and Chase experiment?

1. Radioactive phosphorus in bacterial cells
2. No radioactive sulfur in bacterial cells
3. Both radioactive sulfur and phosphorus in bacterial cells
4. Radioactive sulfur in bacterial cells

Against the codon 5' UAC 3', what would be the sequence of anticodon on tRNA?

1. 5' GUA 3'
2. 5' AUG 3'
3. 5' ATG 3'
4. 5' GTA 3'

If A and C make 30% and 20% of DNA, respectively, what will be the percentage composition of T and G?

1. T : 20%, G : 20%
2. T : 20%, G : 30%
3. T : 30%, G : 20%
4. T : 30%, G : 30%

Complete the flow chart on central dogma. [figure]

1. (a) - Transduction; (b) - Translation; (c) - Replication; (d) - Protein
2. (a) - Replication; (b) - Transcription; (c) - Transduction; (d) - Protein
3. (a) - Translation; (b) - Replication; (c) - Transcription; (d) - Transduction
4. (a) - Replication; (b) - Transcription; (c) - Translation; (d) - Protein

What is the role of RNA polymerase III in the process of transcription in eukaryotes?

1. Transcribes only snRNAs
2. Transcribes rRNAs (28S, 18S and 5.8S)
3. Transcribes tRNA, 5S rRNA and snRNA
4. Transcribes precursor of mRNA

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as

1. Polymorphic DNA
2. Satellite DNA
3. Repetitive DNA
4. Single nucleotides

Identify the correct statement.

1. Split gene arrangement is characteristic of prokaryotes
2. In capping, methyl guanosine triphosphate is added to the 3' end of hnRNA
3. RNA polymerase binds with Rho factor to terminate the process of transcription in bacteria
4. The coding strand in a transcription unit is copied to an mRNA

If Adenine makes 30% of the DNA molecule, what will be the percentage of Thymine, Guanine and Cytosine in it?

1. T : 20 ; G : 25 ; C : 25
2. T : 20 ; G : 30 ; C : 20
3. T : 20 ; G : 20 ; C : 30
4. T : 30 ; G : 20 ; C : 20

Which of the following RNAs is not required for the synthesis of protein?

1. siRNA
2. mRNA
3. tRNA
4. rRNA

Which is the "Only enzyme" that has "Capability" to catalyse Initiation, Elongation and Termination in the process of transcription in prokaryotes?

1. DNase
2. DNA dependent DNA polymerase
3. DNA dependent RNA polymerase
4. DNA Ligase

Which one of the following statements about histones is wrong?

1. Histones carry positive charge in the side chain
2. Histones are organized to form a unit of 8 molecules
3. The pH of histones is slightly acidic
4. Histones are rich in amino acids - Lysine and Arginine

In the light of the above statements, choose the correct answer from the options given below.

1. Statement I is incorrect but Statement II is true
2. Both Statement I and Statement II are true
3. Both Statement I and Statement II are false
4. Statement I is correct but Statement II is false

If the distance between two consecutive base pairs is 0.34 nm and the total number of base pairs of a DNA double helix in a typical mammalian cell is 6.6 109 bp, then the length of the DNA is approximately

1. 2.7 meters
2. 2.0 meters
3. 2.5 meters
4. 2.2 meters

Which of the following statements is correct?

1. Adenine does not pair with thymine
2. Adenine pairs with thymine through one H-bond
3. Adenine pairs with thymine through two H-bonds
4. Adenine pairs with thymine through three H-bonds

The first phase of translation is

1. Recognition of an anti-codon
2. Recognition of DNA molecule
3. Binding of mRNA to ribosome
4. Aminoacylation of tRNA

Name the enzyme that facilitates opening of DNA helix during transcription.

1. DNA polymerase
2. RNA polymerase
3. DNA helicase
4. DNA ligase

Purines found both in DNA and RNA are:

1. Guanine and cytosine
2. Cytosine and thymine
3. Adenine and thymine
4. Adenine and guanine

Expressed Sequence Tags (ESTs) refers to :

1. DNA polymorphism
2. Novel DNA sequences
3. Genes expressed as RNA
4. Polypeptide expression

Under which of the following conditions will there be no change in the reading frame of following mRNA? 5' AACAGCGGUGCUAUU 3'

1. Insertion of A and G at 4th and 5th positions respectively
2. Deletion of GGU from 7th, 8th and 9th positions
3. Insertion of G at 5th position
4. Deletion of G from 5th position

Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology?

1. Genetic code is nearly universal
2. Genetic code is specific
3. Genetic code is not ambiguous
4. Genetic code is redundant

The experimental proof for semi-conservative replication of DNA was first shown in a

1. fungus
2. bacterium
3. plant
4. virus

Select the correct match :

1. Alec Jeffreys - Streptococcus pneumoniae
2. Alfred Hershey and Martha Chase - TMV
3. Matthew Meselson and F. Stahl - Pisum sativum
4. Francois Jacob and Jacques Monod - Lac operon

Select the correct match :

1. Ribozyme - Nucleic acid
2. F2 Recessive parent - Dihybrid cross
3. T.H. Morgan - Transduction
4. G. Mendel - Transformation

Many ribosomes may associate with a single mRNA to form multiple copies of a polypeptide simultaneously. Such strings of ribosomes are termed as

1. polysome
2. polyhedral bodies
3. plastidome
4. nucleosome