Molecular Basis of Inheritance

Which one of the following is wrongly matched?

1. Transcription – Writing information from DNA to t-RNA
2. Operon – Structural genes, operator and promoter
3. Repressor protein – Binds to operator to stop enzyme synthesis
4. Translation – Using information in m-RNA to make protein.

Transformation was discovered by :

1. Griffith
2. Watson and Crick
3. Hershey and Chase
4. Meselson and Stahl

Select the correct option ;

1. Direction of RNA synthesis 3'–5', Direction of reading of the template DNA strand 5'–3'
2. Direction of RNA synthesis 5'–3', Direction of reading of the template DNA strand 5'–3'
3. Direction of RNA synthesis 5'–3', Direction of reading of the template DNA strand 3'–5'
4. Direction of RNA synthesis 3'–5', Direction of reading of the template DNA strand 3'–5'

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

1. Blotting
2. PCR
3. Electrophoresis
4. Autoradiography

Commonly used vectors for human genome sequencing are :

1. BAC and YAC
2. T/A Cloning Vectors
3. T-DNA
4. Expression Vectors

The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C. [figure]

1. A-translation B-extension C-Rosalind Franklin
2. A-translation B-transcription C-Erwin Chargaff
3. A-transcription B-translation C-Francis Crick
4. A-transcription B-replication C-James Watson

Which enzyme will be produced in a cell in which there is a non-sense mutation in the lac Y gene ?

1. Transacetylase
2. Lactose permease and transacetylase
3. Lactose permease
4. -galactosidase

[figure] The figure gives an important concept in the genetic implication of DNA. Fill the blanks A, B and C.

1. A - Erwin Chargaff B - translation C - replication
2. A - Maurice Wilkins B - transcription C - translation
3. A - Francis Crick B - translation C - transcription
4. A - James Watson B - replication C - extension

Which of the following is not a property of the genetic code ?

1. Ambiguous
2. Universal
3. Degeneracy
4. Non-overlapping

In an inducible operon, the genes are :

1. usually expressed unless a signal turns them "off"
2. always expressed
3. never expressed
4. usually not expressed unless a signal turns them "on"

One of the most frequently used techniques in DNA fingerprinting is :-

1. SSCP
2. SCAR
3. VNTR
4. AFLP

Read the following four statements (A-D) : How many of the above statements are right ?

1. Four
2. Two
3. Three
4. One

What is it that forms the basis of DNA Fingerprinting ?

1. The relative proportions of purines and pyrimidines in DNA
2. The relative amount of DNA in the ridges and grooves of the fingerprints
3. The relative difference in the DNA occurrence in blood, skin and saliva
4. Satellite DNA occurring as highly repeated short DNA segments

Removal of introns and joining of exons in a defined order during transcription is called

1. Looping
2. Inducing
3. Slicing
4. Splicing

If one strand of DNA has the nitrogenous base sequence as ATCTG, what would be the complementary RNA strand sequence

1. ATCGU
2. UAGAC
3. AACTG
4. TTAGU

Which one of the following is not a part of a transcription unit in DNA ?

1. The structural gene
2. The inducer
3. A terminator
4. A promoter

Removal of RNA polymerase-III from nucleoplasm will affect the synthesis of

1. tRNA
2. hnRNA
3. rRNA
4. mRNA

PCR and Restriction Fragment Length Polymorphism are the methods for

1. Genetic transformation
2. Genetic fingerprinting
3. Study of enzymes
4. DNA sequencing

The unequivocal proof of DNA as the genetic material came from the studies on a:

1. Viroid
2. Bacterium
3. Fungus
4. Bacterial virus

Which one of the following also acts as a catalyst in a bacterial cell?

1. 5S rRNA
2. snRNA
3. hnRNA
4. 23S rRNA

What are those structures that appear as 'beads-on-string' in the chromosomes when viewed under electron microscope ?

1. Nucleosomes
2. Genes
3. Nucleotides
4. Base pairs

The 3'-5' phosphodiester linkages inside a polynucleotide chain serve to join -

1. One nucleotide with another nucleotide
2. One nitrogenous base with pentose sugar
3. One nucleoside with another nucleoside
4. One DNA strand with the other DNA strand

The lac operon consists of -

1. Three regulatory genes and three structural genes
2. Four regulatory genes only
3. Two regulatory genes and two structural genes
4. One regulatory gene and three structural genes

In eukaryotic cell transcription, RNA splicing and RNA capping take place inside the -

1. Dictyosomes
2. Ribosomes
3. Nucleus
4. ER

Which one of the following statements about the particular entity is true?

1. Nucleosome is formed of nucleotides
2. Centromere is found in animal cells, which produces aster during cell division
3. The gene for producing insulin is present in every body cell
4. DNA consists of a core of eight histones

Which one of the following does not follow the central dogma of molecular biology ?

1. Mucor
2. HIV
3. Chlamydomonas
4. Pea

The one aspect which is not a salient feature of genetic code, is its being –

1. Specific
2. Degenerate
3. Ambiguous
4. Universal

Select the two correct statements out of the four (A–D) statements given below about lac operon. The correct statements are :

1. B and D
2. A and B
3. B and C
4. A and C

Whose experiments cracked the DNA and discovered unequivocally that a genetic code is a "triplet"?

1. Nirenberg and Matthaei
2. Morgan and Sturtevant
3. Hershey and Chase
4. Beadle and Tatum

Semi-conservative replication of DNA was first demonstrated in:

1. Drosophila melanogaster
2. Streptococcus pneumoniae
3. Salmonella typhimurium
4. Escherichia coli